Preliminary phylogenetic identification of virulent Chlamydophila pecorum strains

Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases
Khalil Yousef MohamadAnnie Rodolakis

Abstract

Chlamydophila pecorum is an obligate intracellular bacterium associated with different pathological conditions in ruminants, swine and koala, which is also found in the intestine of asymptomatic animals. A multi-virulence locus sequence typing (MVLST) system was developed using 19 C. pecorum strains (8 pathogenic and 11 non-pathogenic intestinal strains) isolated from ruminants of different geographical origins. To evaluate the ability of MVLST to distinguish the pathogenic from the non-pathogenic strains of C. pecorum, the sequences of 12 genes were analysed: 6 potential virulence genes (ompA, incA, incB, incC, mip and copN), 5 housekeeping genes (recA, hemD, aroC, efp, gap), and the ORF663 gene encoding a hypothetical protein (HP) that includes a variant 15-nucleotides coding tandem repeat (CTR). MVLST provided high discriminatory power (100%) in allowing to distinguish 6 of 8 pathogenic strains in a single group, and overall more discriminatory than MLST targeting housekeeping genes. ompA was the most polymorphic gene and the phylogenetic tree based only on its sequence differentiated 4 groups with high bootstrap values. The number of CTRs (rich in serine, proline and lysine) in ORF663 detected in the pathogenic strains was ...Continue Reading

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Citations

Dec 2, 2009·Applied and Environmental Microbiology·Nadine WinkelmannJens Harder
Dec 24, 2011·Applied and Environmental Microbiology·Jennifer K ParkerLeonardo De La Fuente
May 17, 2011·Journal of Bacteriology·Sergio MojicaGarry S A Myers
Jun 3, 2014·Trends in Microbiology·Nathan L BachmannPeter Timms
Aug 13, 2013·Clinics in Laboratory Medicine·Duncan MacCannell
Apr 7, 2009·Veterinary Microbiology·Annie Rodolakis, Khalil Yousef Mohamad
Jan 9, 2015·Applied Microbiology and Biotechnology·Qingfeng GuanJianhua Wang
Dec 17, 2014·Tropical Animal Health and Production·Salah-Eddine MerdjaKarine Laroucau
Mar 13, 2015·Clinical and Vaccine Immunology : CVI·K Shamsur RahmanBernhard Kaltenboeck
Feb 17, 2021·Veterinary Pathology·Jason D StruthersAlexandra Brower
Apr 14, 2021·Veterinary Microbiology·Lea RohnerNicole Borel
Aug 12, 2014·BMC Genomics·Nathan L BachmannAdam Polkinghorne

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