Preliminary profile of the Cryptosporidium parvum genome: an expressed sequence tag and genome survey sequence analysis

Molecular and Biochemical Parasitology
W B Strong, R G Nelson

Abstract

Cryptosporidium parvum is a protozoan enteropathogen that infects humans and animals and causes a pronounced diarrheal disease that can be life-threatening in immunocompromised hosts. No specific chemo- or immunotherapies exist to treat cryptosporidiosis and little molecular information is available to guide development of such therapies. To accelerate gene discovery and identify genes encoding potential drug and vaccine targets we constructed sporozoite cDNA and genomic DNA sequencing libraries from the Iowa isolate of C. parvum and determined approximately 2000 sequence tags by single-pass sequencing of random clones. Together, the 567 expressed sequence tags (ESTs) and 1507 genome survey sequences (GSSs) totaled one megabase (1 mb) of unique genomic sequence indicating that approximately 10% of the 10.4 mb C. parvum genome has been sequence tagged in this gene discovery expedition. The tags were used to search the public nucleic acid and protein databases via BLAST analyses, and 180 ESTs (32%) and 277 GSSs (18%) exhibited similarity with database sequences at smallest sum probabilities P(N)< or =10(-8). Some tags encoded proteins with clear therapeutic potential including S-adenosylhomocysteine hydrolase, histone deacetylase...Continue Reading

References

Jan 1, 1992·Molecular and Biochemical Parasitology·K KimR G Nelson
Dec 1, 1991·Molecular and Biochemical Parasitology·Fiona TomleyJ J Kok
Jan 1, 1990·Annual Review of Genetics·D A Hopwood, D H Sherman
Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Oct 1, 1990·Protein Engineering·Y Gavel, G von Heijne
Jun 15, 1989·Molecular and Biochemical Parasitology·J G WesselingJ G Schoenmakers
Feb 1, 1986·The Journal of protozoology·W L Current, N C Reese
Feb 1, 1995·International Journal for Parasitology·P J O'Donoghue
Jul 1, 1994·Molecular and Biochemical Parasitology·D ChakrabartiS M Schuster
Jul 1, 1995·Molecular and Biochemical Parasitology·Najib M El-SayedJ E Donelson
Sep 1, 1996·The Journal of Eukaryotic Microbiology·S MalekS Schaaf
Jul 1, 1996·Molecular and Biochemical Parasitology·J B DameE B Werner
Nov 12, 1996·Proceedings of the National Academy of Sciences of the United States of America·Sandra J Darkin-RattrayDennis M Schmatz
Jan 1, 1996·Molecular and Biochemical Parasitology·K L WanJames W Ajioka
Feb 1, 1997·Molecular and Biochemical Parasitology·Najib M El-Sayed, J E Donelson
Sep 1, 1997·Nucleic Acids Research·S F AltschulD J Lipman
Feb 12, 1998·Molecular Microbiology·N V KhramtsovSteve J Upton
Jan 31, 1998·Molecular Microbiology·J M DobrowolskiL D Sibley
Mar 28, 1998·Current Biology : CB·L D SibleyV B Carruthers
Mar 21, 1998·International Journal for Parasitology·J C BoothroydI D Manger
Mar 27, 1998·Biochemical Pharmacology·A L BodleyT A Shapiro
Apr 29, 1998·Advances in Parasitology·J H Crabb
Apr 29, 1998·Advances in Parasitology·H D Ward, A M Cevallos
May 15, 1998·Memórias do Instituto Oswaldo Cruz·A BrandãoW Degrave
Aug 8, 1998·Parasitology·U M MorganR C Thompson
Sep 2, 1998·International Journal for Parasitology·J F DubremetzM N Fourmaux
Sep 2, 1998·International Journal for Parasitology·James W Ajioka
Sep 5, 1998·The Journal of Infectious Diseases·Giovanni WidmerJ K Griffiths
Sep 28, 1998·Biochimica Et Biophysica Acta·E C NenortasT A Shapiro
Oct 15, 1998·Proceedings of the National Academy of Sciences of the United States of America·R F WallerGeoffrey I McFadden
Nov 6, 1998·Molecular and Biochemical Parasitology·M W SmithG A Evans
Jan 9, 1999·Mammalian Genome : Official Journal of the International Mammalian Genome Society·R Y WalderV C Sheffield
Jan 12, 1999·Microbiology·L TetleyGraham H Coombs
Feb 23, 1999·Molecular Microbiology·V B Carruthers, L D Sibley
May 13, 1999·Proceedings of the National Academy of Sciences of the United States of America·M E PerkinsSylvie M Le Blancq

Citations

Jul 17, 2008·Comparative and Functional Genomics·Mitchell S Abrahamsen
Mar 8, 2011·Parasitology Research·Mark C JenkinsRonald Fayer
Jan 29, 2002·International Journal for Parasitology·Ranjit S PaddaPablo C Okhuysen
Jun 14, 2002·International Journal for Parasitology·M J LaGierG Zhu
Apr 11, 2001·Molecular and Biochemical Parasitology·C S JanssenC M Turner
Jan 5, 2002·Molecular and Biochemical Parasitology·Xiaochuan FengGiovanni Widmer
Jan 30, 2002·Molecular and Biochemical Parasitology·Roberta M O'ConnorHonorine D Ward
Feb 19, 2002·Molecular and Biochemical Parasitology·Fernando ZapataSylvie M Le Blancq
Aug 11, 2001·Current Opinion in Immunology·R L Tarleton, Jessica C Kissinger
Aug 23, 2002·Microbes and Infection·Giovanni WidmerMitchell S Abrahamsen
Oct 17, 2002·Trends in Parasitology·Robert P HirtGraham H Coombs
Dec 19, 2003·Nucleic Acids Research·Daniela PuiuJessica C Kissinger
Dec 31, 2005·Nucleic Acids Research·Mark HeigesJessica C Kissinger
Aug 5, 2000·Applied and Environmental Microbiology·X FengGiovanni Widmer
Jul 8, 2005·Applied and Environmental Microbiology·B J KingChristopher P Saint
May 2, 2003·Genome Génome / Conseil National De Recherches Canada·Eline van Zijll de JongJohn W Forster
Apr 9, 2010·BMC Genomics·Sandeep J JosephGerardo R Vasta
Jun 30, 2005·Microbial Cell Factories·Arvind K Bansal
Aug 9, 2001·Proceedings of the National Academy of Sciences of the United States of America·S H KappeV Nussenzweig
Apr 1, 2014·Parasitology·Matthias Lendner, Arwid Daugschies
Mar 15, 2011·Parasitology International·Junya YamagishiXuenan Xuan
Aug 3, 2006·Journal of Biomedicine & Biotechnology·Mehdi Ouaissi, Ali Ouaissi
May 12, 2009·Molecular and Biochemical Parasitology·Chang LiuMitchell S Abrahamsen
Nov 13, 2004·The Journal of Eukaryotic Microbiology·Paul A RochelleKeith Woods
May 21, 2011·Parasite Immunology·Giovanni Widmer, S Sullivan
Jan 25, 2005·Trends in Parasitology·Fiona L HenriquezCraig W Roberts
Nov 24, 2004·Molecular and Biochemical Parasitology·Girija RamakrishnanWilliam A Petri
Mar 1, 2006·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Sultan Tanriverdi, Giovanni Widmer
Sep 18, 2007·Analytical Biochemistry·Philippe ChouvarineDaniel G Peterson
Oct 8, 2004·Journal of Biochemistry and Molecular Biology·Kiew-Lian WanJames W Ajioka
Sep 3, 2003·FEMS Microbiology Letters·Jan SlapetaJanet S Keithly
Jun 15, 2007·FEMS Microbiology Letters·Vlasta CtrnáctáIvan Hrdý
Mar 23, 2012·PloS One·Mary J MauzyMark S Rutherford
Mar 11, 2008·Trends in Parasitology·Saul Tzipori, Giovanni Widmer
Feb 29, 2008·Proteomics·Sanya J SandersonJ M Wastling
Jul 23, 2004·Parasitology·L PutignaniJ M Wastling
Nov 16, 2012·Nature·Martien A M GroenenLawrence B Schook
Jan 4, 2020·The Journal of Eukaryotic Microbiology·Juan David Ospina-VillaLaurence A Marchat
May 10, 2017·Archives of Virology·Minh VongMax L Nibert
Feb 21, 2019·MBio·Lee M YeohStuart A Ralph

Related Concepts

AA167850
AA167851
AA167852
AA167853
AA167854
AA167855
AA167856
AA167857
AA167858
AA167859

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Neural Activity: Imaging

Imaging of neural activity in vivo has developed rapidly recently with the advancement of fluorescence microscopy, including new applications using miniaturized microscopes (miniscopes). This feed follows the progress in this growing field.

The Tendon Seed Network

Tendons are rich in the extracellular matrix and are abundant throughout the body providing essential roles including structure and mobility. The transcriptome of tendons is being compiled to understand the micro-anatomical functioning of tendons. Discover the latest research pertaining to the Tendon Seed Network here.

Myocardial Stunning

Myocardial stunning is a mechanical dysfunction that persists after reperfusion of previously ischemic tissue in the absence of irreversible damage including myocardial necrosis. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Incretins

Incretins are metabolic hormones that stimulate a decrease in glucose levels in the blood and they have been implicated in glycemic regulation in the remission phase of type 1 diabetes. Here is the latest research.

Chromatin Regulation and Circadian Clocks

The circadian clock plays an important role in regulating transcriptional dynamics through changes in chromatin folding and remodelling. Discover the latest research on Chromatin Regulation and Circadian Clocks here.

Long COVID-19

“Long Covid-19” describes illness in patients who are reporting long-lasting effects of the SARS-CoV-19 infection, often long after they have recovered from acute Covid-19. Ongoing health issues often reported include low exercise tolerance and breathing difficulties, chronic tiredness, and mental health problems such as post-traumatic stress disorder and depression. This feed follows the latest research into Long Covid.

Spatio-Temporal Regulation of DNA Repair

DNA repair is a complex process regulated by several different classes of enzymes, including ligases, endonucleases, and polymerases. This feed focuses on the spatial and temporal regulation that accompanies DNA damage signaling and repair enzymes and processes.