Preliminary report on fractionation of fucans by ion-exchange displacement centrifugal partition chromatography

Journal of Chromatography. B, Biomedical Sciences and Applications
L ChevolotC Sinquin

Abstract

A new method combining ion-exchange displacement chromatography with centrifugal partition chromatography (CPC) was used for the fractionation of partially depolymerized fucans (polysulphated polysaccharides). The ion-exchanger was Amberlite LA2, a high-molecular-mass liquid secondary amine miscible with most common organic solvents and immiscible with aqueous solutions. Ion-exchange displacement centrifugal partition chromatography was performed with LA2 in methyl isobutyl ketone (MiBK) as the stationary phase, water as the mobile phase, Cl- as the carrier and OH- as the displacer. A complex mixture of partially depolymerized fucans was resolved into adjacent families characterized by their peak molecular mass and polydispersity. The Dubois test (sugar) and the azur A test (SO3-) confirmed the displacement mode of the process, and size-exclusion chromatographic controls confirmed its efficiency.

References

Oct 15, 1991·Thrombosis Research·S ColliecJ Jozefonvicz
Nov 8, 1996·Journal of Chromatography. a·Y Ito, Y Ma
Aug 1, 1949·The Journal of Physiology·L B JAQUESM STEWART

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Citations

Aug 27, 2013·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Ji-Hyeok LeeYou-Jin Jeon
Mar 23, 2012·Journal of Chromatography. a·Leslie BoudesocqueJean-Hugues Renault

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