Preliminary results in the analysis of the immune response after aneurysmal subarachnoid hemorrhage.

Scientific Reports
Jorge A RoaEdgar A Samaniego

Abstract

Cerebral vasospasm (VSP) is a common phenomenon after aneurysmal subarachnoid hemorrhage (aSAH) and contributes to neurocognitive decline. The natural history of the pro-inflammatory immune response after aSAH has not been prospectively studied in human cerebrospinal fluid (CSF). In this pilot study, we aimed to identify specific immune mediators of VSP after aSAH. Peripheral blood (PB) and CSF samples from patients with aSAH were prospectively collected at different time-points after hemorrhage: days 0-1 (acute); days 2-4 (pre-VSP); days 5-9 (VSP) and days 10 + (post-VSP peak). Presence and severity of VSP was assessed with computed tomography angiography/perfusion imaging and clinical examination. Cytokine and immune mediators' levels were quantified using ELISA. Innate and adaptive immune cells were characterized by flow cytometry, and cell counts at different time-points were compared with ANOVA. Confocal immunostaining was used to determine the presence of specific immune cell populations detected in flow cytometry. Thirteen patients/aneurysms were included. Five (38.5%) patients developed VSP after a mean of 6.8 days from hemorrhage. Flow cytometry demonstrated decreased numbers of CD45+ cells during the acute phase in PB...Continue Reading

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Citations

Nov 30, 2020·Experimental Neurology· Zeyu Zhang Sheng Chen
Jan 13, 2021·Neural Regeneration Research·Junfan Chen, George Kwok Chu Wong
Jul 3, 2021·International Journal of Molecular Sciences·Teodora Larisa TimisIoan Stefan Florian
Sep 1, 2021·Journal of Stroke and Cerebrovascular Diseases : the Official Journal of National Stroke Association·Asad IkramAfshin A Divani

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Methods Mentioned

BETA
Enzyme Linked Immunoassay
flow cytometry
ELISA
PCR

Software Mentioned

GraphPad Prism
BD FACS Diva
Zen

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