Preliminary screening of type IV secretion system in divergent geographic sources of Clostridium difficile

Experimental and Therapeutic Medicine
Na LiWen Zhang

Abstract

In the present study, different geographical sources and sequence types (STs) of Clostridium difficile were preliminarily screened to investigate the distribution profiles of three core genes, VirB4, VirB6 and VirD4, of the type IV secretion system (T4SS). A total of 37 C. difficile strains from different sources were screened, inoculated and prepared for genome extraction. C. difficile toxins A and B were subjected to identification and multilocus sequence typing (MLST) analysis. The T4SS gene then underwent polymerase chain reaction amplification and sequencing analysis. Of the 37 strains, 25 were toxin A- and toxin B-positive, and 12 were toxin A-negative and toxin B-positive. MLST detected 11 strains with ST37, 10 with ST2, 6 with ST35, 7 with ST3, 1 with ST54, 1 with ST1 and 1 with ST119. The detection rates of VirB4, VirB6 and VirD4 were all 100% in colonies exhibiting T4SS. Single nucleotide polymorphisms (SNPs) were detected in a minority of strains. C. difficile strains with identical STs shared the same SNP loci for T4SS, and those with different STs had different SNP loci. The results of the present study may provide evidence for subsequent identification of T4SS distribution, epidemiological investigations, polymorp...Continue Reading

References

Jul 17, 1998·Journal of Clinical Microbiology·H KatoE B Wasito
Oct 10, 2001·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·M Rupnik
Feb 8, 2003·Journal of Clinical Microbiology·Tracy D Wilkins, David M Lyerly
Jan 18, 2005·FEMS Microbiology Reviews·Gil SegalTal Zusman
Sep 13, 2006·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·E J KuijperUNKNOWN European Centre for Disease Prevention and Control
Jul 17, 2007·Trends in Microbiology·Shira Ninio, Craig R Roy
Dec 23, 2008·International Journal of Antimicrobial Agents·Haihui HuangCarl Erik Nord
Apr 7, 2010·Proceedings of the National Academy of Sciences of the United States of America·Miao HeJulian Parkhill
Nov 19, 2010·Lancet·Martijn P BauerUNKNOWN ECDIS Study Group
Feb 25, 2011·Journal of Clinical Microbiology·Ping WangJianguo Xu
Apr 26, 2011·Infection Control and Hospital Epidemiology : the Official Journal of the Society of Hospital Epidemiologists of America·Ying ChengJinxing Lu
Oct 18, 2013·Nature Communications·Michael S M BrouwerPeter Mullany

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