Preliminary studies on development of a novel subunit vaccine targeting Clostridium perfringens mucolytic enzymes for the control of necrotic enteritis in broilers

Poultry Science
A F DuffL R Bielke

Abstract

Necrotic enteritis (NE) is a pervasive enteric disease responsible for large scale economic losses within the global poultry industry. The etiologic agent of NE is Clostridium perfringens (CP), an opportunistic pathogen that utilizes numerous extracellular toxins and glycoside hydrolases (GH) as key virulence and nutrient acquisition factors. Notably, some GH, mucinases, degrade components of mucin in the gastrointestinal tract as an energy source. Targeting this mechanism may serve to reduce the incidence of disease associated with CP. Two experiments were completed that evaluated mucinase vaccine targets sourced from conserved peptide sequences of carbohydrate binding module 32 of CP mucinases. In experiment 1, 37 antigen peptides were synthetically generated and used to produce hyper-immune sera, which was then evaluated for ability to obstruct CP growth in vitro. Total CFU of CP were measured at 4, 6, and 8 h incubation to determine growth rate. Peptides 4, 5, 22, 24, and 30 were selected for further in vivo testing based on conservation or the ability to inhibit CP growth by over 50% at 6 and 8 h. In experiment 2, the aforementioned peptides were conjugated to an agonistic, CD40-targetting antibody and evaluated in vivo. B...Continue Reading

References

Oct 19, 2005·Advances in Parasitology·Martin W ShirleyFiona M Tomley
Oct 28, 2005·Advances in Surgery·William O RichardsRami Lutfi
Dec 11, 2007·Veterinary Immunology and Immunopathology·C T CollierH R Gaskins
Jul 19, 2008·Glycobiology·Dimitris KoutsioulisEllen P Guthrie
May 9, 2009·Journal of Molecular Biology·Elizabeth Ficko-Blean, Alisdair B Boraston
Aug 5, 2011·Avian Pathology : Journal of the W.V.P.A·L TimbermontF Van Immerseel
May 24, 2013·Microbiology and Molecular Biology Reviews : MMBR·Jihong LiBruce A McClane
Jul 2, 2014·Avian Pathology : Journal of the W.V.P.A·Dorien MotFilip Van Immerseel
Oct 11, 2014·Nucleic Acids Research·Randi VitaBjoern Peters
Mar 8, 2016·Avian Pathology : Journal of the W.V.P.A·Jake A LaceyRobert J Moore
May 26, 2017·Osong Public Health and Research Perspectives·Quan-Hoang VuongThu-Trang Vuong
Jul 1, 2017·Frontiers in Veterinary Science·Christine N VuongLuc R Berghman
Dec 14, 2018·BMC Medical Research Methodology·Md Hamidul HuqueKatherine J Lee

❮ Previous
Next ❯

Related Concepts

Related Feeds

Birth Defects

Birth defects encompass structural and functional alterations that occur during embryonic or fetal development and are present since birth. The cause may be genetic, environmental or unknown and can result in physical and/or mental impairment. Here is the latest research on birth defects.