Preliminary studies on the inhibition potential of Indian domestic curd against coliforms, an emerging periodontal pathogen

Journal of Indian Society of Periodontology
Surajit Debnath

Abstract

Coliforms colonize in dental plaques via oral route and may lead to systemic complications.Escherichia coliand its lipopolysaccharide-induced periodontitis is an emerging threat. Clinical management necessitates antibiotic regimens with risk of resistance and upsetting the gut. There is urgent need for better, sustainable, and economical alternative. To investigate the inhibition of coliforms, a potential periodontopathogen directly by Indian domestic curd (IDC) "in situ". Coliforms from natural habitat (Municipal sewage in Agartala, Tripura), a source of infection through food and water, were used as target organism. Domestically prepared curd without any fortification is used to explore its true inhibition potential. Assays of agar well diffusion performed with IDC (ultraviolet sterilized and pH adjusted 6.5) against isolated pure cultures of coliforms. The study protocol nullified effect of organic acids, volatile compounds, bacteriophages, and peroxides in IDC. Peptide nature of inhibitory ingredient was studied by Sodium Dodecyle Sulphate Polyacrylamide Gel Electrophoresis (SDS-PAGE), urea treatment. Computational phylogenetics revealed structural features of inhibitory ingredient. Statistical comparisons were done by anal...Continue Reading

Methods Mentioned

BETA
Electrophoresis

Software Mentioned

Origin
FASTA
ClustalW
MEGA

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