Preliminary study of Cell Wall Structure and its Mechanical Properties of C3H and HCT RNAi Transgenic Poplar Sapling

Scientific Reports
Xianwu ZhouJianxiong Lv

Abstract

This research focused on the cell wall structure and its mechanical properties of down-regulated Coumaroyl shikimate 3-hydroxylase (C3H) transgenic poplar and down-regulated hydroxycinnamoyl CoA: shikimate hydroxycinnamoyl transferase (HCT) transgenic poplar (Populus alba × P. glandulosa cv '84 k'). The wood samples with respect to microstructure, the longitudinal elastic modulus (MOE) and hardness of wood fiber secondary cell wall were investigated. The results show that the lignin contents in the two transgenic poplar woods were lower than non-modified wood. The C3H transgenic poplar and HCT transgenic poplar have more than 18.5% and 16.1% cellulose crystalline regions than non-modified poplar respectively. The diameter of the fiber cell and the vessel element of transgenic poplars are smaller. Double radial vessel cell wall thicknesses of both transgenic poplars were smaller than non-modified poplar. Cell wall ratios for the transgenic poplar were higher than non-modified poplar and cell wall density was significantly lower in both C3H and HCT transgenic poplar. The cell wall MOEs of C3H and HCT transgenic poplar was 5.8% and 7.0% higher than non-modified poplar. HCT can be more effective than C3H to modify the trees by cons...Continue Reading

References

Aug 14, 2001·Plant Physiology·R Hatfield, W Vermerris
Sep 25, 2003·Annual Review of Plant Biology·Wout BoerjanMarie Baucher
Oct 11, 2003·Critical Reviews in Biochemistry and Molecular Biology·Marie BaucherWout Boerjan
May 25, 2006·Plant & Cell Physiology·Shanfa LuVincent L Chiang
Jun 19, 2007·Nature Biotechnology·Fang Chen, Richard A Dixon
Jul 4, 2007·The Plant Journal : for Cell and Molecular Biology·Antanas V SpokeviciusGerd Bossinger
May 15, 2008·The Plant Journal : for Cell and Molecular Biology·Xu LiClint Chapple
Jan 30, 2009·The Plant Journal : for Cell and Molecular Biology·Andrew R Robinson, Shawn D Mansfield
Aug 24, 2010·Biomacromolecules·Sai Venkatesh PingaliBarbara R Evans
Oct 31, 2013·Plant Physiology·Amir MahboubiTotte Niittylä

❮ Previous
Next ❯

Citations

Aug 14, 2019·Frontiers in Plant Science·Alexandra ChanocaWout Boerjan
Sep 2, 2020·The Journal of Biological Chemistry·Nicholas C Carpita, Maureen C McCann
Jun 3, 2020·Trends in Plant Science·Nathan D BryantArthur J Ragauskas

❮ Previous
Next ❯

Methods Mentioned

BETA
transgenic
X-ray
genetic modification
Assay
infrared spectroscopy

Software Mentioned

OMNIC
COMT
CCoAOMT
Image J

Related Concepts