PMID: 11320146Apr 26, 2001Paper

Preliminary study of the genotoxic potential of homocysteine in human lymphocytes in vitro

Mutagenesis
J Crott, M Fenech

Abstract

Homocysteine (Hcy), an immediate precursor of methionine (Met), is considered a risk factor for cardiovascular disease, Alzheimer's disease and neural tube defects. Hcy concentration is also reported to correlate positively with the micronucleus index in lymphocytes in vivo, a marker of chromosome damage. However, it is unclear whether Hcy is genotoxic or simply a biomarker of folate deficiency, a known cause of chromosome damage. We investigated whether high concentrations of Hcy are genotoxic to human lymphocytes in vitro using the cytokinesis-block micronucleus assay. Eighteen lymphocyte cultures were initiated in Met-free and serum-free RPMI 1640 medium for each of four male volunteers aged 22-23 years. At 0, 24, 44 and 72 h, cultures were spiked with L-Hcy or L-Met to achieve concentrations ranging between 50 and 400 microM. The concentration of Hcy at 96 h ranged from 19.45 +/- 2.34 to 149.02 +/- 28.16 microM in Hcy cultures and 0.91 +/- 0.17 to 2.15 +/- 0.9 microM in Met cultures spiked with 50 and 400 microM of metabolite, respectively. Forty-four hours after mitogen stimulation, cytokinesis was inhibited with cytochalasin B. After 96 h, cells were transferred to microscope slides and the frequency of micronucleated-bin...Continue Reading

Citations

Sep 16, 2010·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·A Joan LevineJohn A Baron
Feb 28, 2016·Mutation Research. Genetic Toxicology and Environmental Mutagenesis·Milda StankevičiūtėGintaras Svecevičius
Nov 26, 2009·Journal of Applied Toxicology : JAT·Vilena KasubaZorana Kljaković-Gaspić
Apr 2, 2013·Anesthesiology·Kirk Hogan
Aug 27, 2003·European Journal of Human Genetics : EJHG·Nicoletta BottoAndrea Biagini
Feb 21, 2006·Histopathology·A M W van MarionJ G van den Tweel
Sep 22, 2009·Arquivos brasileiros de endocrinologia e metabologia·Helio Vannucchi, Sandra Soares Melo
Aug 14, 2020·The Science of the Total Environment·José Silonardo Pereira de OliveiraGuilherme Malafaia

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