Preliminary study on conjugation of formononetin with multiwalled carbon nanotubes for inducing apoptosis via ROS production in HeLa cells

Drug Design, Development and Therapy
Bohong GuoJun Yi

Abstract

The present work was conducted to prepare and evaluate multiwalled carbon nanotube-formononetin (MWCNT-FMN) composite for sustained delivery and inducing apop-tosis via reactive oxygen species (ROS) production in HeLa cells. The composite was prepared by solution mixing with short carboxylic group-functionalized multiwalled carbon nanotubes (MWCNT-COOH). Then the composite was characterized by laser particle size analysis, Fourier transform infrared spectrometry, X-ray diffractometry, differential scanning calorimetry, and scanning electron microscopy. Drug release rates in vitro were determined by dialysis method. The in vitro cytotoxicity study was performed using water soluble tetrazolium assay. The cellular apoptosis assay, ROS, and mitochondrial membrane potential (MMP) of HeLa cells were investigated by acridine orange and ethidium bromide double dye, 2',7'-dichlorodihydrofluorescein diacetate, and 5,5',6,6'-tetrachloro-1,1',3,3'-tetraethyl-imidacarbocyanine iodide probe, respectively. The entrapment efficiency was 28.77%±0.15%, and the loading capacity was 12.05%±0.20%. The release of MWCNT-FMN was sustained, and the cumulative release rate of formononetin (FMN) from MWCNT-COOH was higher at pH 7.4 than at pH 5.3. The in...Continue Reading

Citations

May 6, 2019·Cancers·Samantha Kah Ling OngAnupam Bishayee
Aug 14, 2019·Frontiers in Pharmacology·Kai-Ching TayBey-Hing Goh

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Methods Mentioned

BETA
X-ray
scanning electron microscopy
fluorescence microscopy

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis