Preliminary trial: motility comparisons of a unique freezing technology (UFT) to liquid nitrogen mist methodology for cryopreservation of porcine spermatozoa

Reproduction in Domestic Animals = Zuchthygiene
H A GoolsbyS D Prien

Abstract

The motility outcomes of boar semen frozen with newly developed freezing techniques using a new unique freezing technology (UFT) compared with traditional liquid nitrogen methodology were investigated with the intent of improving current fertility outcomes using semen. The UFT is an electronically controlled cooling chamber that houses an organic fluid bath that can be maintained at temperatures below 0 degrees C without solidifying to freeze samples. Four ejaculates from four different boars were collected for this trial. Samples were handled consistently during the pre- and post-freeze processing. From each ejaculate, samples were separated into eight cryopreservation treatment groups, six UFT variations and two control liquid nitrogen groups, immediately before freezing, in replicates of two. After the initial cryopreservation was complete, all samples were stored in liquid nitrogen for at least 48 h. Post-thaw motilities and original motility return percentages were assessed on a random, individual-sample basis. After the initial evaluations, samples from two boars were recollected and frozen using the UFT for breeding purposes. Four sows were bred with the UFT frozen semen to confirm fertility capability. When assessing th...Continue Reading

References

Jun 1, 1978·Cryobiology·K Larsson
Jun 14, 2000·Animal Reproduction Science·M Yoshida
Jun 14, 2000·Animal Reproduction Science·P F Watson
Aug 5, 2000·Animal Reproduction Science·L A JohnsonW M Maxwell
Feb 28, 2002·Reproduction : the Official Journal of the Society for the Study of Fertility·A MedranoW V Holt
Sep 21, 2002·Theriogenology·B M ErikssonH Rodriguez-Martinez

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Citations

Mar 17, 2010·Animal Reproduction Science·N WrenchC E Farin
Apr 12, 2005·Reproduction in Domestic Animals = Zuchthygiene·P Z CotterS D Prien

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