Prenatal alcohol exposure alters phosphorylation and glycosylation of proteins in rat offspring liver

Proteomics
Bourlaye FofanaB L G Nyomba

Abstract

To gain more insights into the translational and PTM that occur in rat offspring exposed to alcohol in utero, 2-D PAGE with total, phospho- and glycoprotein staining and MALDI-MS/MS and database searching were conducted. The results, based on fold-change expression, revealed a down-regulation of total protein expression by prenatal alcohol exposure in 7-day-old and 3-month-old rats. There was an up-regulation of protein phosphorylation but a down-regulation of glycosylation by prenatal alcohol exposure in both age groups. Of 31 protein spots examined per group, differentially expressed proteins were identified as ferritin light chain, aldo-keto reductase, tumor rejection antigen gp96, fructose-1,6-bisphosphatase, glycerol-3-phosphate dehydrogenase, malate dehydrogenase, and gamma-actin. Increased phosphorylation was observed in proteins such as calmodulin, gluthatione S-transferase, glucose regulated protein 58, alpha-enolase, eukaryotic translation elongation factor 1 beta-2, riboprotein large P2, agmatinase, ornithine carbamoyltransferase, quinolinate phosphoribosyltransferase, formimidoyltransferase cyclodeaminase, and actin. In addition, glycosylation of adenosine kinase, adenosylhomocysteine hydrolase, and 3-hydroxyanthran...Continue Reading

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Citations

Jan 14, 2012·Alcohol and Alcoholism : International Journal of the Medical Council on Alcoholism·Cleofina Bosco, Eugenia Diaz
Dec 1, 2012·Current Opinion in Cell Biology·Jonathan R Terman, Anna Kashina
Mar 1, 2015·Neurotoxicology and Teratology·Philip J BushnellJohn M Rogers
Jan 21, 2021·Experimental Biology and Medicine·Suresh MishraBl Grégoire Nyomba
Oct 25, 2017·Chemical Research in Toxicology·Ana ContrerasCarmen Pérez-García

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