Aug 26, 1993

Prenatal determination of fetal RhD type by DNA amplification

The New England Journal of Medicine
P R BennettJ-P Cartron

Abstract

An RhD-negative woman whose partner is heterozygous may have preexisting anti-RhD antibodies that may or may not affect a subsequent fetus, depending on whether it is heterozygous. A safe method of determining fetal RhD type early in pregnancy would eliminate the risks to an RhD-negative fetus of fetal-blood sampling or serial amniocenteses. We determined the RhD type in 15 fetuses using the polymerase chain reaction in amniotic cells and serologic methods in fetal blood collected simultaneously. In another 15 fetuses, the RhD type determined from chorionic-villus samples was compared with that identified by typing of DNA from the fetus itself. RhD typing of DNA from amniotic cells correctly indicated the serologic type in every fetus. Of 10 fetuses with RhD-negative mothers, 4 were identified as RhD-negative and 6 as RhD-positive. Of five fetuses with RhD-positive mothers, four were identified as RhD-positive and one as RhD-negative. There was also complete agreement between the results of RhD typing of DNA from chorionic-villus samples and the results of typing of DNA from fetal tissue. Eleven fetuses were RhD-positive, and 4 were RhD-negative. Four RhD-positive fetuses had RhD-negative mothers. Three RhD-negative fetuses had...Continue Reading

  • References19
  • Citations118

References

Mentioned in this Paper

Blood Grouping and Crossmatching
Intestinal Villus
Embryopathies
Entire Fetus
Collection of Blood Specimen for Laboratory Procedure
Antigen D, Rh Blood Group
Antenatal Screening Procedures
Polymerase Chain Reaction Analysis
Villus
Alloimmunisation

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