Prenatal exposure to carbon monoxide delays postnatal cardiac maturation

Laboratory Investigation; a Journal of Technical Methods and Pathology
Laura SartianiElisabetta Cerbai

Abstract

Prenatal exposure to toxicants, such as maternal smoking, may impair cardiovascular autonomic maturation in infants. We recently showed that exposure of pregnant rats to a mild concentration of carbon monoxide (CO), a component of cigarette smoke, delays postnatal electrophysiological maturation of ventricular myocytes from newborns rats, likely predisposing to life-threatening arrhythmias. To get a comprehensive view of developmental molecular abnormalities induced, at cardiac level, by prenatal CO exposure, we used microarray analysis approach on the rat heart at 4, 7 and 20 days postnatal life. The relationship between molecular and functional alterations was investigated by assessing the ventricular expression of f-current, an electrophysiological marker of immature cardiac phenotype. Rats were prenatally exposed to 0 (CTR) or 150 p.p.m. CO and mRNA obtained from ventricular samples. Differential analysis and biological pathway analysis of microarray data were performed by using Newton's approach and the GENMAPP/MAPPFinder, respectively. The real-time RT-PCR reactions were performed by TaqMan probe-based chemistry. Freshly isolated patch-clamped ventricular cardiomyocytes were used to measure I(f). Genes and pathways contro...Continue Reading

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Citations

Oct 14, 2011·European Journal of Epidemiology·Hanneke Bakker, Vincent W V Jaddoe
Jul 19, 2013·Pediatric Research·Marianne T Neary, Ross A Breckenridge
Oct 26, 2013·International Journal of Epidemiology·H Rob TaalVincent W V Jaddoe

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