Prenatal inflammatory effects on nigrostriatal development in organotypic cultures

Brain Research
Abigail Snyder-Keller, Peri F Stark

Abstract

Maternal intrauterine infection, and the accompanying inflammation in the fetal brain, represent a significant risk to the developing fetus. Dopamine (DA) neurons have been shown to be particularly vulnerable to inflammation induced by injection of the bacterial endotoxin lipopolysaccharide (LPS). In order to further examine the nature of this vulnerability, we used a combination of in vivo prenatal LPS exposure, and in vitro analysis of nigrostriatal development in organotypic cultures prepared from LPS-exposed rat fetuses. Control co-cultures prepared from unexposed E14 substantia nigra (SN/VTA) and E21 striatum exhibited numerous DA neurons in the nigral piece and robust ingrowth into the striatal piece. When E14 SN/VTA was obtained from fetuses exposed to LPS (0.1 mg/kg) on E10, initial DA cell numbers and striatal innervation in co-cultures were normal, but at longer durations in vitro, a reduction in DA neurons was observed. When striatal tissue from fetuses exposed to LPS on E14 or E18 was used in combination with non-exposed SN/VTA, DA neurons initially exhibited a normal pattern of ingrowth into LPS-exposed striatum. However, with longer durations in vitro, DA neurons were lost more rapidly when co-cultured with LPS-ex...Continue Reading

References

Oct 19, 1999·Brain Research. Brain Research Reviews·R Berger, Y Garnier
Feb 22, 2003·The Journal of Comparative Neurology·Ishar DalmauBernardo Castellano
Feb 28, 2004·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Dave A GayleMichael G Ross
Nov 9, 2004·Experimental Neurology·Zaodung LingPaul M Carvey
Oct 25, 2007·Biochemical Society Transactions·M L Block, J-S Hong
Apr 26, 2008·Synapse·Abigail Snyder-KellerPatricio O'Donnell

❮ Previous
Next ❯

Citations

Sep 24, 2010·Anesthesia and Analgesia·Scott Segal
Oct 15, 2014·Nature Reviews. Neurology·Irene KnueselEric P Prinssen
Sep 8, 2009·Brain, Behavior, and Immunity·Insa HoffmannChristiane E L Dammann
Aug 21, 2013·Translational Neuroscience·Sarah E Canetta, Alan S Brown
Sep 7, 2016·Brain, Behavior, and Immunity·Wyston C PierreGregory A Lodygensky
Apr 17, 2020·Frontiers in Pharmacology·Pia M Vidal, Rodrigo Pacheco

❮ Previous
Next ❯

Related Concepts

Related Feeds

Basal Ganglia

Basal Ganglia are a group of subcortical nuclei in the brain associated with control of voluntary motor movements, procedural and habit learning, emotion, and cognition. Here is the latest research.