Prenucleosomes and Active Chromatin

Cold Spring Harbor Symposia on Quantitative Biology
Mai T KhuongJ T Kadonaga

Abstract

Chromatin consists of nucleosomes as well as nonnucleosomal histone-containing particles. Here we describe the prenucleosome, which is a stable conformational isomer of the nucleosome that associates with ∼80 bp DNA. Prenucleosomes are formed rapidly upon the deposition of histones onto DNA and can be converted into canonical nucleosomes by an ATP-driven chromatin assembly factor such as ACF. Different lines of evidence reveal that there are prenucleosome-sized DNA-containing particles with histones in the upstream region of active promoters. Moreover, p300 acetylates histone H3K56 in prenucleosomes but not in nucleosomes, and H3K56 acetylation is found at active promoters and enhancers. These findings therefore suggest that there may be prenucleosomes or prenucleosome-like particles in the upstream region of active promoters. More generally, we postulate that prenucleosomes or prenucleosome-like particles are present at dynamic chromatin, whereas canonical nucleosomes are at static chromatin.

References

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Feb 1, 1979·Nucleic Acids Research·E J Schlaeger, R Knippers
Feb 5, 1980·Journal of Molecular Biology·K H KlempnauerR Knippers
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Jan 10, 2004·Methods in Enzymology·Dmitry V Fyodorov, James T Kadonaga
Oct 26, 2011·Proceedings of the National Academy of Sciences of the United States of America·Jorja G HenikoffSteven Henikoff
Aug 14, 2013·PLoS Biology·Christopher R BrownHinrich Boeger
Jun 17, 2015·Proceedings of the National Academy of Sciences of the United States of America·Haruhiko IshiiBing Ren
Dec 19, 2015·Genes & Development·Jia FeiJames T Kadonaga

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Citations

Mar 28, 2017·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Slawomir KubikDavid Shore
Mar 9, 2018·The Journal of Biological Chemistry·Laura L McCulloughTim Formosa
Oct 7, 2017·The Journal of Biological Chemistry·Mai T KhuongJames T Kadonaga
Aug 16, 2017·Genes & Development·Long Vo NgocJames T Kadonaga

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