Abstract
Tumor angiogenesis is receiving increased attention as a prognostic factor and also as a possible target for new anticancer agents. We investigated whether extent of vascular endothelial growth factor (VEGF) mRNA expression correlated with degree of neovascularization, and whether this expression in fine-needle aspirates could be a marker for assessing angiogenic potential of breast tumors. VEGF mRNA expression was semiquantitated by reverse transcriptase-polymerase chain reaction (RT-PCR) followed by Southern blotting. Tumor neovascularization was assessed by immunohistochemical staining with anti-CD31 (PECAM) antibody. There was a positive correlation between degree of neovascularization and semiquantitated VEGF mRNA expression in invasive ductal carcinomas (r2 = 0.346, n = 48, P < 0.05). Extent of VEGF mRNA expression in fine-needle aspirates was closely correlated with that in resected invasive ductal carcinomas equal to or less than 3 cm in size (r2 = 0.874, n = 14, P < 0.05). These data suggest that semiquantitation of VEGF mRNA expression in fine-needle aspirates is useful for assessing angiogenic potential of invasive ductal carcinomas.
References
Nov 7, 1992·Lancet·E R HorakA L Harris
Jan 3, 1991·The New England Journal of Medicine·N WeidnerJ Folkman
Jan 25, 1991·Cell·L A LiottaW G Stetler-Stevenson
Sep 1, 1990·Journal of Clinical Pathology·D V ParumsD Y Mason
Dec 8, 1989·Science·P J KeckD T Connolly
Apr 1, 1981·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·S M HsuH Fanger
Feb 10, 1984·Nucleic Acids Research·P PonteL Kedes
Apr 29, 1993·Nature·K J KimN Ferrara
Jun 1, 1995·Annals of Surgery·K I BlandH J Wanebo
Jan 1, 1995·Human Pathology·L F BrownS J Schnitt
Apr 16, 1994·Lancet·I A RobinsonM W Kissin
Jun 1, 1994·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Y SoiniP Pääkkö
Jan 1, 1993·The Journal of Clinical Investigation·N FerraraA D Levinson
Mar 1, 1996·Surgery·K AnanM Torisu
Mar 15, 1996·Cancer·M ToiT Tominaga
Citations
Mar 5, 2008·Cancer Investigation·F IovinoF De Vita
Dec 21, 2000·Current Oncology Reports·I Wu, M A Moses
Nov 1, 2008·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Katsunobu TawadaOsamu Yokosuka