Preparation and application of internal surface reversed-phase restricted-access material

Se pu = Chinese journal of chromatography
Xiaoyu WuXianhua Wang

Abstract

A new convenient synthetic method was developed for the preparation of restricted-access material belonging to the group of the internal surface reversed-phase supports, which can be used for the direct injection and analysis of complex biological samples such as plasma samples. The supports, owning the function of reversed-phase chromatography, have an alkyl-chain only to the internal surfaces of the porous silica. Polyvinyl alcoholic groups chemically bound to the external surfaces of the porous silica that has the ability of hydrophilicity. Its structure was characterized by elemental analysis and electron microscope observation. The reversed-phase chromatographic performance was evaluated by using different solute probes, including propranolol, atenolol, phenobarbital and carbamazepine. Meanwhile, the comparative study with Merck Company' s ADS columns was carried out to prove capability of removing protein. At the same time, the plasma sample of propranolol was applied for the determination by direct injections. The results showed that the new internal surface reversed-phase restricted-access material owned the capabilities of protein exclusion and reversed-phase stationary, which could come true the double functions of ex...Continue Reading

References

Jan 20, 1995·Journal of Chromatography. B, Biomedical Applications·A van WaardeW Vaalburg
Feb 23, 1999·Journal of Chromatography. B, Biomedical Sciences and Applications·A Weimann, G Bojesen
Nov 23, 2006·Journal of Biochemical and Biophysical Methods·Wayne M Mullett

Related Concepts

High Pressure Liquid Chromatography Procedure
Rexigen
Quso G-32
Chromatography, Reversed-Phase Liquid
Hydrophobicity
Alkylating Agents
Atenolol
Carbamazepine
Laboratory Procedures
Polyvinyl Alcohol

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