Preparation and application of polyvinyl alcohol-decorated cell membrane chromatography for screening anti-osteoporosis components from Liuwei Dihuang Decoction-containing serum

Journal of Separation Science
Can WuYan Zhu

Abstract

Cell membrane chromatography is a powerful tool for screening active components form complex matrices. New cell membrane carriers need to be developed to increase the coverage of cell membranes on the surface of stationary phases, thereby improving cell membrane chromatographic retention. In this work, we prepared polyvinyl alcohol-poly dimethyl diallyl ammonium chloride modified silica gel as a cell membrane carrier. Osteoblast cell was used as cell membrane source, which was widely used to evaluate the osteogenic activity of drugs. The new cell membrane chromatographic stationary phase was used to screen anti-osteoporosis components in Liuwei Dihuang Decoction-containing serum. The chemical structures of the new modified materials were characterized by scanning electronic microscopy, fourier transform infrared spectroscopy and elemental analysis characterization. Compared with the common cell membrane column, the cell membrane coverage of this modified material was increased by 30%, and thus provided a stronger retention effect in positive drugs. 19 metabolites in rat serum samples were retained on the cell membrane chromatography and identified by ultra-high-performance liquid chromatography/time-of-flight mass spectrometry....Continue Reading

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Citations

Feb 20, 2021·Bioscience, Biotechnology, and Biochemistry·Hui LiuMiaokuo Lin

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