Preparation and Binding Evaluation of Histamine-Imprinted Microspheres via Conventional Thermal and RAFT-Mediated Free-Radical Polymerization

ACS Omega
Edwin F RomanoClovia I Holdsworth

Abstract

Elevated histamine (HTM) levels are closely linked to food poisoning as well as to pathophysiological allergic diseases. In this study, HTM-imprinted, solution-processable microspheres were prepared via high-dilution conventional thermal polymerization (CTP) and controlled radical polymerization (CRP) using ethylene glycol dimethacrylate (80 or 90 wt %) and methacrylic acid at 60 °C in acetonitrile and evaluated as recognition materials for sensing applications. The polymers were selective to HTM in binding studies, cross-rebinding, and competitive binding assays against the HTM analogues histidine, imidazole, and tryptamine. The selective binding capacity was significantly higher with CTP-80 (on the basis of mass: 21.0 μmol/g and surface area: 8.08 × 10-2 μmol/m2) than that with both CTP-90 (8.47 μmol/g, 4.49 × 10-2 μmol/m2) and CRP-80 (9.00 μmol/g, 1.19 × 10-2 μmol/m2).

References

Jan 1, 1989·Journal of Toxicology. Clinical Toxicology·S L TaylorJ A Nordlee
Sep 1, 1983·Clinical Reviews in Allergy·D L Marquardt
Mar 20, 2001·JAMA : the Journal of the American Medical Association·K BeckerJ N MacCormack
Feb 20, 2003·Journal of the American Chemical Society·F Javier RamírezEstanislao Silla
Apr 20, 2004·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Robert J UmplebyKen D Shimizu
May 27, 2006·Biosensors & Bioelectronics·Somchai BoonpangrakLei Ye
Jun 15, 2007·Analytical Chemistry·Chun-Hua LuXiao-Ru Wang
Oct 4, 2007·Organic & Biomolecular Chemistry·Adam McCluskeyMichael C Bowyer
Sep 25, 2009·Journal of the American Chemical Society·Arnaud CutivetKarsten Haupt
Apr 12, 2011·Biomacromolecules·Nghia P TruongMichael J Monteiro
Apr 25, 2012·Amino Acids·Foteini A TrikkaDimitrios A Kyriakidis
Sep 17, 2013·Biomacromolecules·Nguyen T D TranMichael J Monteiro

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Citations

Sep 6, 2017·Journal of Molecular Recognition : JMR·Edwin F RomanoRegina C So

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Methods Mentioned

BETA
column chromatography
dynamic light scattering
saturation binding

Software Mentioned

ImageJ
Zetasizer

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