Preparation and characterisation of dual-layer latex-coated columns for open-tubular capillary electrochromatographic preconcentration of cations combined in-line with their separation by capillary electrophoresis

Electrophoresis
Shusheng ZhangP R Haddad

Abstract

A dual-layer ion-exchange latex-coated column was prepared and characterised for on-capillary preconcentration of cations using an open-tubular ion-exchange CEC format. After preconcentration, the analyte cations were eluted with a transient isotachophoretic gradient and separated by CE. The latex double layer was established by first coating the negatively charged wall of the capillary with a layer of cationic quaternary ammonium anion-exchange Dionex AS5A latex particles (60 nm diameter), and then coating a layer of anionic sulphonated cation-exchange Dionex CS3 latex particles (300 nm diameter) onto the underlying AS5A layer. The adhesion of layers is based on electrostatic attractions. Several dual-layer capillaries were characterised for their EOF and ion-exchange capacity and this showed that coatings could be prepared reproducibly by a simple flushing procedure. The dual-layer columns exhibited a moderate, pH-independent EOF (ca. 26 x 10(-9 )m2V(-1)s(-1)) and an ion-exchange capacity of 57 microequiv./g (or 2.69 nequiv./column). Using an 8 cm length of coated capillary combined with a 72 cm length of untreated capillary, a method for on-line preconcentration and separation of monovalent organic bases, alkali metal ions a...Continue Reading

References

Aug 12, 2000·Analytical Chemistry·J ZhangC Horváth
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Citations

Nov 14, 2014·Analytical Chemistry·Bingcheng YangPurnendu K Dasgupta
Nov 10, 2007·Journal of Chromatography. a·Yi ChenChanggui Qiu
Nov 2, 2007·Journal of Separation Science·Violaine AugustinMarie-Claire Hennion
Dec 7, 2007·Electrophoresis·F W Alexander TempelsGerhardus J de Jong
Sep 7, 2007·Electrophoresis·Pavel CoufalKarel Stulík
Dec 29, 2007·Electrophoresis·Yuli Huo, Wim Th Kok
Jan 2, 2007·Journal of Biochemical and Biophysical Methods·L Saavedra, C Barbas
Nov 20, 2012·Talanta·Xuefang DiaoXiaogang Chu
Jul 14, 2017·Electrophoresis·Vojtech Adam, Marketa Vaculovicova
Feb 15, 2017·Analytical Chemistry·Weixiong HuangPurnendu K Dasgupta

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