Preparation and Characterization of Covalently Binding of Rat Anti-human IgG Monolayer on Thiol-Modified Gold Surface.

Nanoscale Research Letters
Zhengjian LvGuoping Chen

Abstract

The 16-mercaptohexadecanoic acid (MHA) film and rat anti-human IgG protein monolayer were fabricated on gold substrates using self-assembled monolayer (SAM) method. The surface properties of the bare gold substrate, the MHA film and the protein monolayer were characterized by contact angle measurements, atomic force microscopy (AFM), grazing incidence X-ray diffraction (GIXRD) method and X-ray photoelectron spectroscopy, respectively. The contact angles of the MHA film and the protein monolayer were 18° and 12°, respectively, all being hydrophilic. AFM images show dissimilar topographic nanostructures between different surfaces, and the thickness of the MHA film and the protein monolayer was estimated to be 1.51 and 5.53 nm, respectively. The GIXRD 2θ degrees of the MHA film and the protein monolayer ranged from 0° to 15°, significantly smaller than that of the bare gold surface, but the MHA film and the protein monolayer displayed very different profiles and distributions of their diffraction peaks. Moreover, the spectra of binding energy measured from these different surfaces could be well fitted with either Au4f, S2p or N1s, respectively. Taken together, these results indicate that MHA film and protein monolayer were succes...Continue Reading

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Citations

Mar 8, 2014·Micron : the International Research and Review Journal for Microscopy·Congzhou Wang, Vamsi K Yadavalli
Sep 4, 2010·International Journal of Biological Macromolecules·Zhengjian LvLinhong Deng
May 18, 2010·Journal of Colloid and Interface Science·Nan Cheng, Xudong Cao
Mar 22, 2012·Bioconjugate Chemistry·Rong WangLiaohai Chen

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