Preparation and characterization of ethanol-treated silk fibroin dense membranes for biomaterials application using waste silk fibers as raw material

Bioresource Technology
Grínia M NogueiraMarisa Masumi Beppu

Abstract

The possibility of producing valued devices from low cost natural resources is a subject of broad interest. The present study explores the preparation and characterization of silk fibroin dense membranes using waste silk fibers from textile processing. Morphology, crystallinity, thermal resistance and cytotoxicity of membranes as well as the changes on the secondary structure of silk fibroin were analyzed after undergoing treatment with ethanol. Membranes presented amorphous patterns as determined via X-ray diffraction. The secondary structure of silk fibroin on dense membranes was either random coil (silk I) or beta-sheet (silk II), before and after ethanol treatment, respectively. The sterilized membranes presented no cytotoxicity to endothelial cells during in vitro assays. This fact stresses the material potential to be used in the fabrication of biomaterials, as coatings of cardiovascular devices and as membranes for wound dressing or drug delivery systems.

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Citations

Nov 22, 2013·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Mariana Agostini de MoraesMarisa Masumi Beppu
Nov 6, 2015·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Wing P ChanMeng-Yi Bai
Apr 19, 2017·Materials Science & Engineering. C, Materials for Biological Applications·Qiongyu WangJiannan Wang
Jan 30, 2015·Brazilian Oral Research·Rafael Menezes SilvaAna Márcia Macedo Ladeira Carvalho
May 27, 2014·Materials Science & Engineering. C, Materials for Biological Applications·Mariana Agostini de MoraesMarisa Masumi Beppu
Dec 4, 2014·Biomacromolecules·Mariana Agostini de MoraesMarisa Masumi Beppu
Jul 28, 2015·Macromolecular Bioscience·Raul MachadoMargarida Casal
Jan 1, 2018·Polymers·Fangfang TuJiannan Wang
Oct 28, 2016·Journal of Materials Chemistry. B, Materials for Biology and Medicine·N DrnovšekS Novak
Dec 22, 2020·Frontiers in Bioengineering and Biotechnology·Melissa PuertaAdriana Restrepo-Osorio
Dec 2, 2020·Materials Science & Engineering. C, Materials for Biological Applications·Bruna Gregatti de CarvalhoLucimara Gaziola de la Torre
Feb 12, 2021·International Journal of Biological Macromolecules·D V Santhosh Kumar GunapuSiva Rama Krishna Vanjari
Mar 2, 2021·Materials Science & Engineering. C, Materials for Biological Applications·Sanaz KhademolqoraniFatemeh Ajalloueian
Apr 7, 2021·Journal of Microbiology and Biotechnology·Gicheol KwonSung Sun Park
May 1, 2021·Biomedical Physics & Engineering Express·Aisling M MannTraian Chirila

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