Preparation and characterization of excretory and secretory antigen of Cotylophoron cotylophorum and Gastrothylax crumenifer

Journal of Parasitic Diseases : Official Organ of the Indian Society for Parasitology
G AravindhanS Gomathinayagam

Abstract

Excretory and secretory antigen of adult Cotylophoron cotylophorum and Gastrothylax crumenifer was prepared and characterized by SDS-PAGE. Adult paramphistomes were collected in PBS from rumen of sheep slaughtered at Perambur and Villivakkam slaughter houses, Chennai, Tamil Nadu. Excretory/Secretory (E/S) antigens of G. crumenifer and C. cotylophorum were prepared by incubating in PBS at 28.3 °C overnight in a shaking incubator and centrifuged. The culture supernatant was used as antigen. The protein concentration of E/S antigens of C. cotylophorum and G. crumenifer ranged from 0.4 to 7.1 mg/ml and 3.3 to 5.9 mg/ml, respectively. SDS-PAGE analysis of E/S antigen of C. cotylophorum revealed two polypeptide bands from the range of 32 and 40 kDa. Whereas, SDS-PAGE analysis of E/S antigen of G. crumenifer revealed only one polypeptide band of 35 kDa. Excretory/secretory antigen may be used for serodiagnosis of paramphistomosis infection in sheep.

References

Jan 1, 1971·Advances in Parasitology·I G Horak
Mar 24, 2000·Veterinary Parasitology·M K SaifullahS M Abidi
Mar 30, 2007·Cardiovascular Drugs and Therapy·Jonathan AfilaloMark J Eisenberg
Sep 26, 2007·Experimental Parasitology·Panat AnuracpreedaPrasert Sobhon
Apr 5, 2011·Veterinary Parasitology·Mohammad K SaifullahSyed M A Abidi
Oct 17, 2014·Journal of Parasitic Diseases : Official Organ of the Indian Society for Parasitology·R GodaraAnkur Rastogi

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