PMID: 16535847Mar 16, 2006Paper

Preparation and characterization of immunogen against microcystin LR

Wei sheng yan jiu = Journal of hygiene research
Xiao-lian ZhaoJian Tang

Abstract

To prepare Microcystins-LR (MC-LR) conjugates with the carrier protein for developing a new immune assay for MC-LR. MC-LR was coupled to BSA or KLH by "activated ester" and "water-soluble carbodimide". Measure the combine ratio of MC-LR with KLH or BSA by the UV scanning or the Bradford's method. The results are 9.1 and 9.4. New Zealand rabbits was immunized by MC-LR-KLH. The titer of the polyantibodies was over 25,600 measured by id ELISA. MC-LR-KLH was proved the suitable immunogen against MC-LR.

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