Preparation and characterization of mixed-mode monolithic silica column for capillary electrochromatography

Journal of Chromatography. a
Fanggui YeShulin Zhao

Abstract

A silica-based monolithic stationary phase with mixed-mode of reversed phase (RP) and weak anion-exchange (WAX) for capillary electrochromatography (CEC) has been prepared. The mixed-mode monolithic silica column was prepared using the sol-gel technique and followed by a post-modification with hexadecyltrimethoxysilane (HDTMS) and aminopropyltrimethoxysilane (APTMS). The amino groups on the surface of the stationary phase were used to generate a substantial anodic EOF as well as to provide electrostatic interaction sites for charged compounds at low pH. A cathodic EOF was observed at pH above 7.3 due to the full ionization of residual silanol groups and the suppression in the ionization of amino groups. A variety of analytes were used to evaluate the electrochromatographic characterization and column performance. The monolithic stationary phase exhibited RP chromatographic behavior toward neutral solutes. The model anionic solutes were separated by the mixed-mode mechanism, which comprised RP interaction, WAX, and electrophoresis. Symmetrical peaks can be obtained for basic solutes because positively charged amino groups can effectively minimize the adsorption of positively charged analytes to the stationary phase.

References

Sep 10, 2004·Journal of Chromatography. a·Emily F HilderJean M J Fréchet
May 23, 2006·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Karel StulíkPavel Coufal
Apr 10, 2007·Journal of Chromatography. a·Mohamed GuerrouacheMarie-Claude Millot
Mar 4, 2008·Journal of Chromatography. a·Oscar NúñezNobuo Tanaka

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Citations

May 29, 2016·Journal of Pharmaceutical and Biomedical Analysis·Kelly Zhang, Xiaodong Liu
Sep 21, 2016·Journal of Pharmaceutical and Biomedical Analysis·Kelly Zhang, Xiaodong Liu
Jul 10, 2014·The Analyst·Sheng TangShengxiang Jiang
Mar 16, 2013·Journal of Separation Science·Qiaoli ZhouLi Jia
Oct 29, 2011·Journal of Chromatography. a·Yun Yang, Xindu Geng

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