PMID: 2095206Jan 1, 1990Paper

Preparation and characterization of paramagnetic polychelates and their protein conjugates

Bioconjugate Chemistry
P F SievingS M Rocklage

Abstract

The gadolinium complexes of poly-L-lysine-poly(diethylenetriamine-N,N,N',N",N"-pentaacetic acid) (Gd-PL-DTPA) and poly-L-lysine-poly(1,4,7,10-tetraazacyclododecane-N,N',N",N"'-tetr aacetic acid) (Gd-PL-DOTA) and their conjugates with human serum albumin (HSA) have been prepared and characterized. Poly-L-lysine (PL, degree of polymerization approximately 100) was N-acylated with a mixed anhydride of the chelating ligand (DTPA or DOTA). Sixty to ninety chelating groups per molecule of PL could be attached in this way. Following purification of the polychelate by size-exclusion chromatography, the gadolinium complexes were prepared by standard methods and conjugated to HSA with heterobifunctional cross-linking reagents. The molar relaxities of these macromolecular species were 2-3-fold higher than those of the corresponding monomeric metal complexes [( Gd(DTPA)] and [Gd(DOTA)]). The conjugation conditions were optimized to produce conjugates containing 60-90 metal centers per molecule of HSA (ca. one polychelate per protein).

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Citations

Jan 1, 1994·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·E C WienerP C Lauterbur
Jan 1, 1994·Breast Cancer Research and Treatment·K Orang-KhadiviR F Williams
Nov 20, 2012·Journal of the American Chemical Society·Eszter BorosPeter Caravan
Mar 16, 2013·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Mazen JamousWalter Mier
May 11, 2011·Advanced Drug Delivery Reviews·Hua Ai
Jan 22, 2009·Contrast Media & Molecular Imaging·Carlos F G C Geraldes, Sophie Laurent
Sep 19, 2006·Journal of Magnetic Resonance·Huiming Zhang, Yang Xie
Jun 1, 2006·Journal of Magnetic Resonance·Huiming Zhang, Yang Xie
Jul 27, 2006·Technology in Cancer Research & Treatment·Egidijus E UzgirisStephen Lomnes
Apr 13, 2004·Investigative Radiology·Egidijus Uzgiris
Sep 1, 1994·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·M F WendlandC B Higgins
Jul 1, 1993·Journal of Magnetic Resonance Imaging : JMRI·J VymazalR A Brooks
Sep 21, 2005·Angewandte Chemie·Zhaoda ZhangPeter Caravan
Feb 21, 2013·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Abdolrasoul SoleimaniElizabeth R Gillies
Mar 9, 2011·Chemical Society Reviews·Luciano LattuadaLorenzo Tei
Jan 18, 2007·Bioconjugate Chemistry·Sibu BalanSteve Brocchini
Aug 2, 2005·Molecular Pharmaceutics·Vincent J VendittoMartin W Brechbiel
May 18, 2006·Bioconjugate Chemistry·Anurag MishraNikos K Logothetis
Dec 13, 2007·Bioconjugate Chemistry·Luis M De León-Rodríguez, Zoltan Kovacs
Apr 21, 2001·Bioconjugate Chemistry·M G DuarteC F Geraldes
Feb 15, 2001·Bioconjugate Chemistry·S Liu, D S Edwards

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