Preparation and characterization of small-diameter decellularized scaffolds for vascular tissue engineering in an animal model

Biomedical Engineering Online
Shuangyue XuZhongquan Qi

Abstract

The development of a suitable extracellular matrix (ECM) scaffold is the first step in vascular tissue engineering (VTE). Synthetic vascular grafts are available as an alternative to autologous vessels in large-diameter arteries (>8 mm) and medium-diameter arteries (6-8 mm). In small-diameter vessels (<6 mm), synthetic vascular grafts are of limited use due to poor patency rates. Compared with a vascular prosthesis, natural tissue ECM has valuable advantages. Despite considerable progress in recent years, identifying an optimal protocol to create a scaffold for use in small-diameter (<6 mm) fully natural tissue-engineered vascular grafts (TEVG), remains elusive. Although reports on different decellularization techniques have been numerous, combination of and comparison between these methods are scarce; therefore, we have compared five different decellularization protocols for making small-diameter (<6 mm) ECM scaffolds and evaluated their characteristics relative to those of fresh vascular controls. The protocols differed in the choice of enzymatic digestion solvent, the use of non-ionic detergent, the durations of the individual steps, and UV crosslinking. Due to their small diameter and ready availability, rabbit arteria caro...Continue Reading

References

Jan 23, 1998·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·M S Conte
Dec 30, 1998·The Annals of Thoracic Surgery·H B Barner
Apr 16, 1999·Science·L E NiklasonR Langer
Jul 12, 2001·Annual Review of Biomedical Engineering·R M Nerem, D Seliktar
Jun 14, 2002·Medical Engineering & Physics·B S ConklinC Chen
Nov 22, 2002·European Journal of Vascular and Endovascular Surgery : the Official Journal of the European Society for Vascular Surgery·A J KovalicA H Davies
Jan 30, 2003·Vascular Medicine·Briain D MacNeillJoseph P Vacanti
Feb 5, 2004·European Journal of Vascular and Endovascular Surgery : the Official Journal of the European Society for Vascular Surgery·J SchmidliT P Carrel
May 25, 2004·Annals of Vascular Surgery·Robert L MaddenAlexander Kurbanov
Feb 25, 2005·Annals of Surgery·Seung-Woo ChoByung-Soo Kim
Mar 24, 2005·European Journal of Cardio-thoracic Surgery : Official Journal of the European Association for Cardio-thoracic Surgery·Robert W GraussMarco C DeRuiter
Jul 4, 2006·Journal of Biomedical Materials Research. Part a·Steven R MeyerDavid B Ross
Jul 6, 2006·European Urology·Gerhard FeilKarl-Dietrich Sievert
Aug 17, 2010·Acta Biomaterialia·A YahyoucheA J P Clover
Feb 8, 2011·Biomaterials·Peter M CrapoStephen F Badylak
May 17, 2011·Proceedings of the National Academy of Sciences of the United States of America·Clay QuintLaura E Niklason
May 24, 2011·International Journal of Biological Macromolecules·Ping XiangZhi-hua Zhou
Oct 15, 2011·Cells, Tissues, Organs·Marissa PeckNicolas L'Heureux
Mar 10, 2012·Journal of the Mechanical Behavior of Biomedical Materials·W S SheridanB P Murphy
Jan 11, 2013·Liver International : Official Journal of the International Association for the Study of the Liver·Haozhen RenYitao Ding
Jul 13, 2013·Tissue Engineering. Part a·Heidi DebelsKeren Abberton
Oct 24, 2013·Wiley Interdisciplinary Reviews. Systems Biology and Medicine·Song LiShu Chien
Jan 16, 2014·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Wanyin ZhaiJiang Chang
Jan 22, 2014·Circulation·Alan S GoUNKNOWN American Heart Association Statistics Committee and Stroke Statistics Subcommittee
Oct 9, 2015·Tissue Engineering. Part B, Reviews·Samand Pashneh-TalaFrederik Claeyssens
Nov 30, 2016·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·F StarneckerN Thierfelder
Sep 1, 2003·Cell Transplantation·Shannon L M DahlLaura E Niklason

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Citations

Nov 6, 2018·Journal of Biomedical Materials Research. Part a·Esin Akbay, Mehmet Ali Onur
Mar 7, 2019·Macromolecular Bioscience·Jinge LiXiaoniu Yang
Jan 11, 2019·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Randall J SmithStelios T Andreadis
Oct 28, 2019·Journal of Biomedical Materials Research. Part a·Haoyong YuanZhongshi Wu
Feb 6, 2020·Journal of Tissue Engineering and Regenerative Medicine·Aurore B Van de WallePeter S McFetridge
May 12, 2020·Tissue Engineering and Regenerative Medicine·Soo Yeon JungHan Su Kim
Sep 30, 2020·The International Journal of Artificial Organs·Zhijuan HeNing Li
Apr 3, 2020·Nature Communications·Randall J SmithStelios T Andreadis
May 29, 2018·Trends in Molecular Medicine·Thomas Colunga, Stephen Dalton
May 27, 2021·Biotechnology and Bioengineering·Senthilkumar MuthusamyTong Cao
Nov 26, 2021·Frontiers in Bioengineering and Biotechnology·Mingzhe SongTao Qian

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Methods Mentioned

BETA
Assay
xenograft

Software Mentioned

Motic Image Advanced
GraphPad

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