PMID: 6252346Sep 1, 1980Paper

Preparation and characterization of specific antisera to individual glycoprotein antigens comprising the major glycoprotein region of herpes simplex virus type 1

Journal of Virology
R Eberle, R J Courtney

Abstract

The major glycoprotein complex (VP123) of herpes simplex virus type 1 resolved by sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis was purified and further fractionated into two major and two minor components by chromatography of the isolated VP123 region on SDS-hydroxylapatite columns. The two major components (gC and gA/gB) were purified free of other polypeptides and used to prepare specific antisera to these glycoproteins. Radioimmune precipitation demonstrated that these antisera were specific for the antigens used in their production. These two antisera as well as an anti-VP123 serum were further characterized by immunoprecipitation, neutralization, and membrane immunofluorescence techniques. Results indicate that both of the major glycoprotein antigens are expressed on the surface of virions as well as on the surface of infected cells.

References

Dec 1, 1978·International Journal of Cancer. Journal International Du Cancer·P SethD Ghosh
Jun 1, 1970·American Journal of Epidemiology·A J NahmiasB A Guest
Oct 1, 1972·Virology·U Olshevsky, Y Becker
Jul 1, 1970·Proceedings of the National Academy of Sciences of the United States of America·J A Reynolds, C Tanford

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Citations

Jan 1, 1995·Archives of Virology·R EberleJ K Hilliard
Jan 1, 1989·Archives of Virology·J K HilliardR Eberle
Apr 1, 1986·Journal of Virology·M W Wathen, L M Wathen
Jun 1, 1985·Journal of Virology·N Balachandran, L M Hutt-Fletcher
Feb 1, 1987·Journal of Virology·M GongE Kieff
May 1, 1993·Journal of Virology·H K SuR J Courtney
Apr 1, 1983·Journal of Virology·E A Wenske, R J Courtney
Nov 1, 1994·Journal of Virology·R B TenserK A Hay

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