PMID: 9434343Jan 22, 1998Paper

Preparation and characterization of the F (ab)2 fragments of an aromatase activity-suppressing monoclonal antibody

Steroids
P C Ng, Y Osawa

Abstract

The preparation and characterization of the Fab and F(ab')2 fragments of a murine monoclonal antibody specific for aromatase cytochrome P-450 and which is suppressive of estrogen biosynthesis are described. This monoclonal antibody, MAb3-2C2, was purified from murine ascites using protein A affinity chromatography and digested with immobilized papain to produce antibody fragments. The Fab and F(ab')2 fragments were then purified using protein A affinity chromatography and S-200 HR size exclusion chromatography. The Fab fragment was further purified using S-100 HR size exclusion chromatography. Both the Fab and F(ab')2 fragments of the MAb3-2C2 suppressed aromatase activity in a dose-dependent manner. While the F(ab')2 fragment (110 kDa) maintained potent suppressive activity, the Fab fragment (42 kDa) required a higher concentration to suppress aromatase activity as compared to the IgG.

References

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Citations

Dec 28, 1999·Analytical Biochemistry·M B DainiakB Mattiasson
May 28, 2003·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Vladimir I Muronetz, Timo Korpela
Jul 22, 2008·Analytical Biochemistry·Jun Pyo KimSang Jun Sim
Jan 20, 2009·Journal of Immunological Methods·Pete GagnonPaul J Yazaki
May 27, 2004·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Camelia I SpiridonEllen S Vitetta

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