Preparation and characterization of vinculin-targeted polymer-lipid nanoparticle as intracellular delivery vehicle

International Journal of Nanomedicine
Junping WangXiaojun Yu

Abstract

Intracellular delivery vehicles have been extensively investigated as these can serve as an effective tool in studying the cellular mechanism, by delivering functional protein to specific locations of the cells. In the current study, a polymer-lipid nanoparticle (PLN) system was developed as an intracellular delivery vehicle specifically targeting vinculin, a focal adhesion protein associated with cellular adhesive structures, such as focal adhesions and adherens junctions. The PLNs possessed an average size of 106 nm and had a positively charged surface. With a lower encapsulation efficiency 32% compared with poly(lactic-co-glycolic) acid (PLGA) nanoparticles (46%), the PLNs showed the sustained release profile of model drug BSA, while PLGA nanoparticles demonstrated an initial burst-release property. Cell-uptake experiments using mouse embryonic fibroblasts cultured in fibrin-fibronectin gels observed, under confocal microscope, that the anti-vinculin conjugated PLNs could successfully ship the cargo to the cytoplasm of fibroblasts, adhered to fibronectin-fibrin. With the use of cationic lipid, the unconjugated PLNs were shown to have high gene transfection efficiency. Furthermore, the unconjugated PLNs had nuclear-targeting ...Continue Reading

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Citations

Methods Mentioned

BETA
light scattering
Protein Assay
transfection
confocal
light-scattering

Related Concepts

Texas red
LactoSorb
Drug Carriers
Fibroblasts
Immunofluorescence Microscopy
Particle Size
Biofix
Serum Albumin, Bovine
Xanthenes
Isovinculin

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