Preparation and Characterization of Electrostatically Crosslinked Polymer⁻Liposomes in Anticancer Therapy

International Journal of Molecular Sciences
Yi-Ting ChiangChun-Liang Lo

Abstract

pH-sensitive polymer⁻liposomes can rapidly release their payloads. However, it is difficult to simultaneously achieve stability and pH-responsiveness in the polymer⁻liposomes. In this study, stable and pH-sensitive crosslinked polymer⁻liposomes were fabricated through electrostatic interactions. The pH-sensitive copolymer methoxy poly(ethylene glycol)-block-poly(methacrylic acid)-cholesterol (mPEG-b-P(MAAc)-chol) and crosslinking reagent poly(ethylene glycol) with end-capped with lysine (PEG-Lys2) were synthesized and characterized. At physiological conditions, the pH-sensitive copolymers were anionic and interacted electrostatically with the cationic crosslinker PEG-Lys2, forming the electrostatically-crosslinked polymer⁻liposomes and stabilizing the liposomal structure. At pH 5.0, the carboxylic groups in mPEG-b-P(MAAc)-chol were neutralized, and the liposomal structure was destroyed. The particle size of the crosslinked polymer⁻liposomes was approximately 140 nm and the polymer⁻liposomes were loaded with the anticancer drug doxorubicin. At pH 7.4, the crosslinked polymer⁻liposomes exhibited good stability with steady particle size and low drug leakage, even in the presence of fetal bovine serum. At pH 5.0, the architecture o...Continue Reading

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Citations

Jan 18, 2020·Current Cancer Drug Targets·Kuldeep Rajpoot
Apr 5, 2020·Nanomaterials·Shi Su, Peter M Kang
Jul 3, 2020·International Journal of Biological Macromolecules·Avinash Singh PatelPraveen Kumar Sappati

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Methods Mentioned

BETA
dynamic laser scattering
PC-C
electrophoretic light scattering
ELISA

Software Mentioned

Excel

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