PMID: 444575Apr 18, 1979

Preparation and chemical characterisation of calf Tamm-Horsfall glycoprotein

Biochimica Et Biophysica Acta
W van DijkW Ferwerda

Abstract

Tamm-Horsfall glycoprotein preparations were obtained from calf urine by 1.0 M NaCl precipitation followed by 4 M urea/Sepharose 4B chromatography. By using 0.1% sodium dodecyl sulfate polyacrylamide gel electrophoresis a molecular weight of 86 500 +/- 4500 (n = 12) was calculated for the glycoprotein. Amino acid and carbohydrate analyses were performed, the carbohydrate composition being (in residues per 100 amino acid residues in the glycoprotein): fucose, 0.90; galactose, 4.82; mannose, 4.63;N-acetylglucosamine, 7.36; N-acetylgalactosamine, 1.38; sialic acid, 2.93. Under conditions of mild acid hydrolysis (0.05 M H2SO4, 80 degrees C, 1 h) the calf Tamm-Horsfall glycoprotein preparations were degraded partially into two lower molecular weight fragments (approximate Mr 66 000 and 51 000), as shown by polyacrylamide gel electrophoresis, both fragments being periodic acid-Schiff reagent positive.

References

Jul 30, 1974·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·D R DunstanA Neuberger
Jan 1, 1972·Annual Review of Biochemistry·R D Marshall
May 1, 1950·Proceedings of the Society for Experimental Biology and Medicine·I TAMM, F L HORSFALL

Citations

Jan 1, 1997·Urological Research·J A GokhaleS R Khan
Jan 1, 1991·Virchows Archiv. A, Pathological Anatomy and Histopathology·Y NagashimaK Misugi
May 1, 1981·Developmental Biology·P EkblomL Saxén
Jan 1, 1983·Clinical and Experimental Hypertension. Part A, Theory and Practice·J Greven
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Apr 12, 2005·Clinical Biochemistry·Jean DubePierre Douville
Jan 11, 1991·The Journal of Urology·D E NealM B Kaack

Related Concepts

Acetylgalactosamine
Acetylglucosamine
Metazoa
Bos indicus
Monosaccharides
Mucoproteins
Sialic Acids

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