Preparation and chromatographic evaluation of a newly designed steviol glycoside modified-silica stationary phase in hydrophilic interaction liquid chromatography and reversed phase liquid chromatography

Journal of Chromatography. a
Tu LiangXinmiao Liang

Abstract

A diterpene glycoside compound, rebaudioside A (commonly abbreviated as RA), was immobilized onto porous silica surface through "thiol-ene" click chemistry strategy. The successful immobilization of the RA on the silica support was confirmed by FT-IR and elemental analysis. Chromatographic characteristics of the new stationary phase, named Click TE-RA, were evaluated by a set of diverse analytes such as carbohydrates, nucleosides, and organic acids in hydrophilic interaction liquid chromatography (HILIC) mode. The effects of water content, buffer pH and concentration were investigated and a typical HILIC retention feature of Click TE-RA was observed at high organic modifier content. The Click TE-RA stationary phase was further studied by a series of glycoside compounds. Tunable retention mechanisms from hydrophilic to hydrophobic interactions were observed. Separation of very polar compounds including oligosaccharides, nucleic acid bases and nucleosides using Click TE-RA in HILIC mode was successfully accomplished. In addition, separation of saponins both in HILIC and reversed-phase liquid chromatography (RPLC) modes was performed, demonstrating the presence of orthogonality between two different modes on Click TE-RA column. Th...Continue Reading

References

Sep 15, 2006·Journal of Separation Science·Petrus Hemström, Knut Irgum
Jun 15, 2007·Chemical Communications : Chem Comm·Zhimou GuoXinmiao Liang
Nov 12, 2009·Chemical Communications : Chem Comm·Hongxue HuangXinmiao Liang
Mar 29, 2011·Journal of Chromatography. a·Andreas E KaratapanisConstantine D Stalikas
Jul 9, 2011·Journal of Chromatography. a·Yong Guo, Sheetal Gaiki
Aug 2, 2011·Journal of Chromatography. a·Ngoc Phuoc DinhKnut Irgum
Aug 13, 2013·Journal of Chromatography. a·Andrea F G GarganoMichael Lämmerhofer
Dec 29, 2013·Journal of Chromatography. a·Xiujie GuoXinmiao Liang

❮ Previous
Next ❯

Citations

Oct 16, 2015·Journal of Chromatography. a·Xianzhe ShiGuowang Xu
Apr 17, 2015·Journal of Pharmaceutical and Biomedical Analysis·Balázs MagdaPál T Szabó
Mar 7, 2020·Journal of Separation Science·Hao ZhangYang Chen
Jun 19, 2020·International Journal of Molecular Sciences·Jolanta FliegerMałgorzata Tatarczak-Michalewska

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cardiac Glycosides

Cardiac glycosides are a diverse family of naturally derived compounds that bind to and inhibit na+/k+-atpase. Discover the latest research on cardiac glycosides heres.