Preparation and evaluation of amphipathic lipopeptide-loaded PLGA microspheres as sustained-release system for AIDS prevention

Engineering in Life Sciences
Huijuan JinGuanghui Ma

Abstract

At present, AIDS drugs are typical inhibitors that cannot achieve permanent effects. Therefore, the research of blocking HIV infection is essential. Especially for people in the high-risk environment, long-term prevention is important, because HIV can easily infect cells once the drug is interrupted. However, there is still no long-acting AIDS prevention drug approved. Hence, the purpose of this study is to prepare a fusion inhibitor loaded poly(d, l-lactic-co-glycolic acid) (PLGA) microspheres as a sustained-release system for long-term AIDS prevention. As the HIV membrane fusion inhibitor (LP-98) used in this research is amphiphilic lipopeptide, W1/O/W2 double-emulsion method was chosen, and premix membrane emulsification technique was used for controlling the uniformity of particle size. Several process parameters that can impact drug loading efficiency were summarized: the concentration of LP-98 and PLGA, and the preparation condition of primary emulsion. Finally, the microspheres with high loading efficiency (>8%) and encapsulation efficiency (>90%) were successfully prepared under optimum conditions. Pharmacokinetic studies showed that LP-98-loaded microspheres were capable to continuously release for 24 days in rats. Thi...Continue Reading

References

Jan 24, 2012·International Journal of Pharmaceutics·Deepak N KapoorSanju Dhawan
Jan 31, 2012·Intervirology·Saleta Sierra-Aragón, Hauke Walter
Aug 11, 2012·The Journal of Biological Chemistry·Huihui ChongYuxian He
Sep 26, 2012·Antimicrobial Agents and Chemotherapy·Todd J JohnsonPatrick F Kiser
Dec 25, 2013·Antimicrobial Agents and Chemotherapy·Sam N RothsteinSteven R Little
Aug 16, 2014·International Journal of Pharmaceutical Investigation·Josephine Leno JenitaBarnabas Wilson
Sep 1, 2015·Journal of Controlled Release : Official Journal of the Controlled Release Society·Antoinette G NelsonPatrick J Sinko
Oct 6, 2015·Annals of Internal Medicine·Michael J SilverbergUNKNOWN North American AIDS Cohort Collaboration on Research and Design of the International Epidemiologic Databases to Evaluate AID
Jul 19, 2016·The New England Journal of Medicine·Myron S CohenUNKNOWN HPTN 052 Study Team
Aug 28, 2016·Journal of Controlled Release : Official Journal of the Controlled Release Society·Keiji HirotaSteven P Schwendeman
Aug 27, 2017·Scientific Reports·Killian QuinnAlan Winston
Mar 15, 2018·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Parker W Lee, Jonathan K Pokorski
Feb 28, 2019·ACS Infectious Diseases·Terumasa IkedaBrian R McNaughton
Apr 29, 2019·European Journal of Medicinal Chemistry·Devashish SenguptaRitu Gaur
Jul 16, 2019·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Tengteng WangRongcai Liang

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Citations

Mar 27, 2021·Advances in Colloid and Interface Science·Akmal Nazir, Goran T Vladisavljević

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