Preparation and evaluation of Baicalin-loaded cationic solid lipid nanoparticles conjugated with OX26 for improved delivery across the BBB

Drug Development and Industrial Pharmacy
Zhidong LiuNan Li

Abstract

A novel brain targeting drug delivery system based on OX26 antibody conjugation on PEGylated cationic solid lipid nanoparticles (OX26-PEG-CSLN) was prepared. The Baicalin-loaded PEGylated cationic solid lipid nanoparticles modified by OX26 antibody (OX26-PEG-CSLN) were prepared by emulsion evaporation-solidification at low temperature method. The immune-gold labeled OX26-PEG-CSLN was visualized by transmission electron microscopy. The mean diameter and zeta potential of OX26-PEG-CSLN, PEG-CSLN and CSLN were determined using a Zetasizer. The entrapment efficiency of OX26-PEG-CSLN, PEG-CSLN and CSLN was determined by ultrafiltration centrifugation method. And the solid-state characterization of OX26-PEG-CSLN and CSLN were analyzed by X-ray. Pharmacokinetics studies were conducted by in vivo microdialysis in rat cerebrospinal fluid. The results showed that the OX26-PEG-CSLN, PEG-CSLN and CSLN had average diameters of 47.68 ± 1.65, 27.20 ± 1.70 and 33.89 ± 5.74 nm, Zeta potentials of -0.533 ± 0.115 mV, 11.200 ± 0.500 mV and 11.080 ± 1.170 mV and entrapment efficiencies of 83.03 ± 0.01%, 92.90 ± 3.50% and 97.83 ± 0.19%, respectively. In the pharmacokinetics studies, the AUC value of OX26-PEG-CSLN was11.08-fold higher than that of th...Continue Reading

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Citations

Feb 9, 2016·Journal of Pharmaceutical Sciences·Maria GregoriEmanuela Cazzaniga
Mar 10, 2017·Drug Development and Industrial Pharmacy·Haohuan LiTianyuan Ci
Jun 27, 2017·Journal of Controlled Release : Official Journal of the Controlled Release Society·Reham S ElezabyGehanne A Awad
Jan 1, 2016·BioImpacts : BI·Jaleh BararYadollah Omidi
Sep 14, 2018·European Journal of Drug Metabolism and Pharmacokinetics·Ting HuangChengliang Zhang
Dec 4, 2019·Drug Delivery and Translational Research·Shimon Ben-ShabatArik Dahan
Jun 13, 2018·Brain Sciences·Kandhasamy SowndhararajanSongmun Kim
Aug 16, 2017·The Journal of Pharmacy and Pharmacology·Shuya WangZhidong Liu
Aug 4, 2019·Pharmaceutics·Robert AncuceanuVlad Negulescu
Dec 23, 2020·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Shilpi SinghSuaib Luqman

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