Preparation and Imaging Investigation of Dual-targeted C3 F8 -filled PLGA Nanobubbles as a Novel Ultrasound Contrast Agent for Breast Cancer

Scientific Reports
Jing DuFeng-Hua Li

Abstract

Molecularly-targeted contrast enhanced ultrasound (US) imaging is a promising imaging strategy with large potential for improving diagnostic accuracy of conventional US imaging in breast cancer detection. Therefore, we constructed a novel dual-targeted nanosized US contrast agent (UCA) directed at both vascular endothelial growth factor receptor 2 (VEGFR2) and human epidermal growth factor receptor 2 (HER2) based on perfluoropropane (C3F8)-filled poly(lactic-co-glycolic acid) (PLGA) (NBs) for breast cancer detection. In vitro, single- or dual-targeted PLGA NBs showed high target specificities and better effects of target enhancement in VEGFR2 or HER2-positive cells. In vivo, US imaging signal in the murine breast cancer model was significantly higher (P < 0.01) for dual-targeted NBs than single-targeted and non-targeted NBs. Small animal fluorescence imaging further confirmed the special affinity of the dual-targeted nanosized contrast agent to both VEGFR2 and HER2. Immunofluorescence and immunohistochemistry staining confirmed the expressions of VEGFR2 and HER2 on tumor neovasculature and tumor cells of breast cancer. In conclusions, the feasibility of using dual-targeted PLGA NBs to enhance ultrasonic images is demonstrated i...Continue Reading

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Citations

Jun 27, 2019·Journal of Ultrasound in Medicine : Official Journal of the American Institute of Ultrasound in Medicine·Christopher LauVinay Duddalwar
Sep 16, 2020·Biomaterials Science·Andy WijayaGuixue Wang
Apr 18, 2020·Frontiers in Pharmacology·Hiroshi KidaKatsuro Tachibana
Oct 2, 2020·Nanomaterials·Gurbet KöseFabian Kiessling
Jun 8, 2021·Advanced Functional Materials·Mehdi AziziMichael R Hamblin
Jun 14, 2021·Advances in Colloid and Interface Science·Marie Pierre Krafft, Jean G Riess
Feb 11, 2020·Langmuir : the ACS Journal of Surfaces and Colloids·Ananda J Jadhav, Mostafa Barigou

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Methods Mentioned

BETA
dynamic light scattering
confocal microscopy
fluorescence-activated cell sorter
fluorescence imaging
xenograft

Software Mentioned

Win MDI
DigSubAna
SPSS

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