Preparation and in vitro evaluation of meloxicam-loaded PLGA nanoparticles on HT-29 human colon adenocarcinoma cells

Drug Development and Industrial Pharmacy
C Tuba Şengel-TürkNurşin Gönül

Abstract

The inhibitors of cyclooxygenase (COX)-2 play an important role in cancer chemoprevention. Certain COX-2 inhibitors exert antiproliferative and pro-apoptotic effects on cancer cells. In this study, meloxicam, which is an enolic acid-type preferential COX-2 inhibitor, was encapsulated in poly(D,L-lactide-co-glycolide) (PLGA) nanoparticles (NPs) to maintain local high concentration, and its efficacy was determined. NPs were prepared by using salting-out and emulsion-evaporation steps. Meloxicam-loaded NP formulations were evaluated with respect to the drug loading, particle size, polydispersity index, zeta potential, drug release rate, and residual poly(vinyl alcohol) (PVA) percentage. The effects of PLGA and PVA molecular weight variations on the physicochemical properties of NPs were investigated. Stability of meloxicam in NPs was assessed over 3 months. COX-2 expressing human colon adenocarcinoma cell line HT-29 was used in cellular uptake and viability assays. NPs had a spherical shape and a negative zeta potential, and their size ranged between 170-231 nm with a lower polydispersity index. NPs prepared with high molecular weight PLGA were shown to be physically stable over three months at 4°C. The increase in molecular weigh...Continue Reading

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Citations

Apr 10, 2013·Drug Development and Industrial Pharmacy·Huayan SunGuiyang Liu
Jul 16, 2014·Drug Development and Industrial Pharmacy·Xu BaoYan Tian
Oct 28, 2016·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Shalaka DhatBirendra Shrivastava
Nov 20, 2018·Frontiers in Pharmacology·Sima RezvantalabFabian Kiessling
Oct 13, 2018·Small Methods·Jia ZhuangLiangfang Zhang
Apr 24, 2021·Medicinal Research Reviews·Firdos A KhanFaheem H Pottoo

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