Preparation and in vivo study of dry powder microspheres for nasal immunization

Journal of Drug Targeting
Mohsen Tafaghodi, Somayeh Rastegar

Abstract

The immunoadjuvant potential of alginate microspheres as delivery system, and cross-linked dextran microspheres (CDM) as absorption enhancer and excipient for powder of alginate microspheres, were evaluated. Alginate microspheres were prepared by emulsification method. Microspheres encapsulated with tetanus toxoid (TT) or Quillaja saponin (QS) were nasally administered to rabbits, three times in 2 weeks interval and serum IgG and nasal lavage sIgA titers were determined by ELISA. The mean diameter of microspheres was about 1.5 mum. Release of TT and QS was 13.1 +/- 1.4% and 31.8 +/- 4.3% after 4 h. The serum IgG titer induced with (TT)(ALG) microspheres was higher than TT solution (P<0.001). Addition of QS or CDM adjuvant, in separate, to (TT)(ALG) microspheres could not significantly increase the immune responses (P>0.05), but the highest systemic IgG titers induced with (TT+QS)(ALG)+CDM (P<0.01). The sIgA titer induced with (TT)(ALG) microspheres was higher than TT solution (P<0.05). The highest mucosal sIgA titers were seen in animals immunized with (TT)(ALG)+CDM (P<0.05). Co-encapsulation of QS and TT in microspheres did not increase the sIgA titers. When CDM was added to alginate microspheres encapsulated with TT or TT+QS,...Continue Reading

References

Jan 1, 1995·Pharmaceutical Biotechnology·C R KensilS Soltysik
Sep 1, 1996·Immunological Investigations·R M CarrN L O'Sullivan
Jun 6, 2000·Advanced Drug Delivery Reviews·L Pereswetoff-Morath
Jun 7, 2000·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·P JohansenB Gander
Jul 22, 2004·International Journal of Pharmaceutics·Mohsen TafaghodiMahdi Momen-Nejad
Nov 25, 2004·Advanced Drug Delivery Reviews·H Oya AlparV W Bramwell
Sep 27, 2005·Advanced Drug Delivery Reviews·Michael I UgwokeRenaat Kinget
Nov 1, 2005·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·I J GillL Illum
Jun 27, 2006·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Mohsen TafaghodiSayyed Abolghasem Sajadi Tabassi
Sep 26, 2006·Methods : a Companion to Methods in Enzymology·Caroline D Skene, Philip Sutton
Jan 10, 2008·AAPS PharmSciTech·Robert J GarmiseAnthony J Hickey
Feb 26, 2008·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Akhlesh Kumar JainPrakash V Diwan
Jun 10, 2008·International Journal of Pharmaceutics·S Abolghasem Sajadi TabassiMahmoud Reza Jaafari
Jan 6, 2009·Advanced Drug Delivery Reviews·Noemi CsabaMaria Jose Alonso
Feb 12, 2009·Vaccine·Hong-Xiang SunYi-Ping Ye
Jun 30, 2009·Vaccine·Xiaoming Song, Songhua Hu
Aug 29, 2009·Journal of Controlled Release : Official Journal of the Controlled Release Society·Niels HagenaarsWim Jiskoot

❮ Previous
Next ❯

Citations

Mar 22, 2013·AAPS PharmSciTech·Ragwa M FaridAbd El Azeem R Ebian
Nov 3, 2010·Journal of Drug Targeting·Barbara C Baudner, Derek T O'Hagan
Feb 11, 2016·Journal of Controlled Release : Official Journal of the Controlled Release Society·Akhilesh Kumar ShakyaHarvinder Singh Gill
Feb 25, 2014·International Journal of Pharmaceutics·Seyed Salman Razavi RohaniMohsen Tafaghodi
Feb 2, 2017·Therapeutic Delivery·Nirmal MarasiniIstvan Toth
Nov 30, 2018·Expert Opinion on Drug Delivery·Regina Scherließ
Jan 10, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Juliane Deise FleckSimone Gasparin Verza
Jul 27, 2018·Advances in Pharmacological Sciences·Silvy CherianGregory Smutzer
Oct 20, 2018·Journal of Controlled Release : Official Journal of the Controlled Release Society·Sachin G ThakkarZhengrong Cui

❮ Previous
Next ❯

Related Concepts

Related Feeds

Avian Influenza: Innate Immune Adjuvant (ASM)

Adjuvants systems that are added to vaccines against avian influenza have be explored to enhance the innate immune system response against the virus. Here is the latest research on avian influenza and the innate immune adjuvant.

CREs: Gene & Cell Therapy

Gene and cell therapy advances have shown promising outcomes for several diseases. The role of cis-regulatory elements (CREs) is crucial in the design of gene therapy vectors. Here is the latest research on CREs in gene and cell therapy.

Avian Influenza: Innate Immune Adjuvant

Adjuvants systems that are added to vaccines against avian influenza have be explored to enhance the innate immune system response against the virus. Here is the latest research on avian influenza and the innate immune adjuvant.

Related Papers

Journal of Controlled Release : Official Journal of the Controlled Release Society
Cynthia BosquillonRita Vanbever
Drug Discovery Today. Technologies
Morten Jonas Maltesen, Marco van de Weert
Journal of Controlled Release : Official Journal of the Controlled Release Society
Suzanne M BalJoke A Bouwstra
© 2022 Meta ULC. All rights reserved