Preparation and in vivo/in vitro evaluation of formononetin phospholipid/vitamin E TPGS micelles

Journal of Drug Targeting
Xudong ChengZhenhai Zhang

Abstract

To enhance the formononetin (FN) antitumor effect, we developed a passive targeting FN-contained formulation. FN-contained Vitamin E d-α-tocopheryl polyethylene glycol 1000 succinate (vitamin E TPGS or TPGS)/phospholipid micelles were prepared by the solvent injection method. Particle size, polydispersity, zeta potential, encapsulation efficiency, drug release profile, and micelles morphology were evaluated and characterized by various methods including high-performance liquid chromatography, dynamic light scattering, and transmission electron microscopy. Cellular uptake of micelles was evaluated with fluorescence imaging coupled with HPLC method. Cytotoxicity of FN micelles and free FN was compared using MTT method. In vivo imaging was employed to assess the accumulation of DiR micelles and free DiR at tumor site. The antitumor effect of FN micelles was examined in tumor-bearing mice. The results showed that prepared FN micelles had an average particle diameter of 111.91 ± 5.82 nm with good stability. FN micelles enhanced the cellular uptake and improved cell cytotoxicity than free FN. Furthermore, DiR micelles quickly accumulated at the tumor site than free DiR. FN micelles significantly improved tumor inhibition rate compare...Continue Reading

References

May 16, 1998·Proceedings of the National Academy of Sciences of the United States of America·S K HobbsR K Jain
Aug 9, 2002·Journal of Agricultural and Food Chemistry·William H TollesonDean W Roberts
Mar 5, 2008·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Kwangjae ChoDong M Shin
Nov 6, 2008·Journal of Biomedical Materials Research. Part a·Haizheng Zhao, Lin Yue Lanry Yung
Feb 21, 2009·Molecular Pharmaceutics·Anne-Katrin KirchherrKarsten Mäder
Feb 13, 2010·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Mathieu GoutayerIsabelle Texier
Mar 11, 2010·Handbook of Experimental Pharmacology·Vladimir P Torchilin
Apr 13, 2010·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·M Wang, M Thanou
Jun 29, 2010·Annals of Oncology : Official Journal of the European Society for Medical Oncology·L CrinòUNKNOWN ESMO Guidelines Working Group
Jan 27, 2011·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Sheelendra Pratap SinghGirish Kumar Jain
Sep 21, 2011·Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Métabolisme·J ChenX Chen
Oct 18, 2011·International Journal of Pharmaceutics·Madaswamy S MuthuSi-Shen Feng
Feb 14, 2012·Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Métabolisme·Y YeZ Mo
Apr 14, 2012·Biomaterials·Zhiping ZhangSi-Shen Feng
Nov 20, 2012·European Journal of Drug Metabolism and Pharmacokinetics·Daniela Cristina VitaleSalvatore Salomone
Mar 6, 2013·Expert Opinion on Drug Delivery·Anandhkumar RajuSi-Shen Feng
Jul 23, 2013·Chemico-biological Interactions·Yu-Li Lo, Wanjen Wang
Nov 26, 2013·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Yue-mei JinMan-hua Cui
Jul 1, 2014·Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Métabolisme·R ZhouH Chen
Aug 30, 2014·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Yun LiuZengMing Xiao
Sep 24, 2014·International Journal of Pharmaceutics·Fabienne DanhierVéronique Préat
Oct 11, 2014·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Tianyu LiYu Ye
Dec 3, 2014·International Journal of Pharmaceutics·Xin-Jiang TangJian-Qing Gao
Dec 3, 2014·International Journal of Pharmaceutics·Yongtai ZhangNianping Feng

❮ Previous
Next ❯

Citations

Aug 26, 2016·Expert Opinion on Drug Delivery·Omkara Swami MuddinetiSwati Biswas
Dec 18, 2018·Pharmaceutical Development and Technology·Lijun ZhenJiangnan Yu
Aug 14, 2019·Frontiers in Pharmacology·Kai-Ching TayBey-Hing Goh
Jul 20, 2021·Critical Reviews in Food Science and Nutrition·Hui TengLei Chen

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.