Preparation and maintenance of organotypic slice cultures of CNS tissue

Current Protocols in Neuroscience
B H GähwilerD Muller

Abstract

Organotypic slice cultures are the in vitro method of choice for applications requiring long-term survival of the preparation and a high degree of cellular differentiation and organization resembling that of the original tissue. Long-term survival is achieved by culturing slices at the air/liquid interface, either by continuously rotating the preparation (roller-tube cultures) or by culturing them on semiporous membranes (stationary interface cultures). Both culture techniques yield nerve cells which are highly differentiated in terms of their morphological and physiological characteristics. Because most of these cultures are prepared from 1-week-old postnatal animals, in which the cellular and tissue organization is already relatively advanced, the original cytoarchitecture is often remarkably well maintained. Moreover, the presence of a full complement of glial and nerve cells is thought to provide a microenvironment facilitating differentiation of neurons. Slice culture also offers unique advantages for recording from pairs of cells, as a consequence of the high degree of connectivity between nerve cells. Recently, new applications have emerged such as the cultivation of slices from knock-out animals with limited postnatal s...Continue Reading

References

Apr 1, 1991·Journal of Neuroscience Methods·L StoppiniD Muller
Apr 1, 1990·Journal of Neuroscience Methods·C M AnnisR T Robertson
Nov 1, 1988·Trends in Neurosciences·B H Gähwiler
Sep 20, 1984·The Journal of Comparative Neurology·J Zimmer, B H Gähwiler
Dec 1, 1981·Journal of Neuroscience Methods·B H Gähwiler
Jan 15, 1993·Brain Research. Developmental Brain Research·P A BuchsD Muller
Jan 15, 1993·Brain Research. Developmental Brain Research·D MullerL Stoppini
Nov 5, 1997·Trends in Neurosciences·B H GähwilerS M Thompson

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Citations

Jun 16, 2011·Journal of Visualized Experiments : JoVE·Robert C FoehringAngela R Cantrell
Apr 12, 2016·Frontiers in Neuroscience·Nathaniel J KillianJelena Vukasinovic
Mar 13, 2019·Current Protocols in Neuroscience·Zhihui Huang, Ying Wang
Nov 17, 2012·Current Protocols in Stem Cell Biology·Eric HerleniusPetri Olivius

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