PMID: 2506011Aug 15, 1989Paper

Preparation and properties of a cross-linked complex between ferredoxin--NADP+ reductase and flavodoxin

European Journal of Biochemistry
J J PueyoC Gomez-Moreno

Abstract

The electrostatically stabilized complex between Anabaena variabilis ferredoxin--NADP+ reductase and Azotobacter vinelandii flavodoxin has been covalently cross-linked by treatment with 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide. The covalent complex exhibits a molecular mass and FMN/FAD content consistent with that expected for a 1:1 stoichiometry of the two flavoproteins. Immunochemical cross-reactivity is exhibited by the covalent complex with rabbit antisera prepared separately against each protein. The complex retains NADPH-ferricyanide diaphorase activity although the Km for ferricyanide is increased twofold and the turnover number is decreased by a factor of two when compared to native reductase. NADPH-cytochrome-c reductase activity of the complex is observed at a level that is quite similar to that determined at saturating concentrations of flavodoxin, while it is only 1-2% of that exhibited by the reductase in the presence of ferredoxin. No stimulation of cytochrome-c reductase activity is observed on adding ferredoxin to the cross-linked complex. Stopped-flow data show that covalent cross-linking of the flavodoxin to the reductase reduces the rate of electron transfer from its semiquinone form to cytochrome c by a...Continue Reading

References

Sep 8, 1965·Biochemical and Biophysical Research Communications·R M Smillie
Oct 10, 1968·Biochemical and Biophysical Research Communications·M Shin, A S Pietro
Oct 15, 1971·Biochemical and Biophysical Research Communications·M A Cusanovich, D E Edmondson
Jan 1, 1972·Archiv für Mikrobiologie·B Van Lin, H Bothe
Jan 25, 1968·Biochemical and Biophysical Research Communications·N Nelson, J Neumann
Jan 1, 1981·Annual Review of Nutrition·A H MerrillD B McCormick

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Citations

Oct 1, 1993·Photosynthesis Research·J J Pueyo, C Gómez-Moreno
Jun 16, 2009·The FEBS Journal·Emanuela BalconiAlessandro Aliverti
Jul 1, 1991·Archives of Biochemistry and Biophysics·J Sancho, C Gómez-Moreno
Sep 9, 2000·Biochemistry·D A HallR G Matthews
Sep 3, 1990·Biochimica Et Biophysica Acta·M F FillatC Gomez-Moreno
May 1, 1992·Archives of Biochemistry and Biophysics·J J PueyoC Gómez-Moreno

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