PMID: 6167677Aug 1, 1981Paper

Preparation and properties of an immunosorbent column specific for the myelin basic protein

Journal of Neurochemistry
L S ReidlA T Campagnoni

Abstract

An immunosorbent column specific for the myelin basic protein (BP) was prepared by coupling purified anti-BP antibodies to cyanogen bromide (BrCN)-activated Sepharose 4B. The BP-immunosorbent column bound BP between pH 4.5 and pH 6.8. In its working range the column bound approximately 400-475 microgram of BP at pH 6.8 and 250 microgram at pH 4.5 with recoveries of 72-77%. The BP-immunosorbent column could effectively separate BP from simple mixtures of BP and proteins of similar size and charge and from acid extracts of bovine brain. The results indicate that the BP-immunosorbent column can be used to isolate BP from a mixture of proteins and may be adapted for use in the small-scale purification of the myelin basic proteins involving a minimum number of steps.

References

Oct 1, 1975·Journal of Neurochemistry·S R CohenM Guarnieri
Sep 9, 1975·Biochimica Et Biophysica Acta·L F LiebesW D Phillips
Aug 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·E BarbareseJ H Carson
Sep 1, 1978·Archives of Biochemistry and Biophysics·C W CampagnoniA T Campagnoni
Jan 1, 1974·Methods in Enzymology·D M Livingston
Oct 1, 1974·Immunochemistry·E D Day, O M Pitts
Mar 1, 1969·Archives of Biochemistry and Biophysics·S Panyim, R Chalkley

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Citations

Apr 1, 1993·Journal of Neuroscience Research·K Asotra, W B Macklin
Sep 1, 1993·Journal of Neuroscience Research·S Amur-UmarjeeA T Campagnoni
Nov 1, 1994·Journal of Neuroscience Research·E C SchirmerS Szuchet
May 1, 1987·Neurochemical Research·J E MoskaitisA T Campagnoni
Nov 26, 1982·Journal of Immunological Methods·B Karlsson, C Alling
Jul 1, 1983·Journal of Neuroscience Methods·L S Reidl, A T Campagnoni
Jan 1, 1991·Neurochemistry International·K NakajimaK Miyamoto
Jun 1, 1994·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·S Byravan, A T Campagnoni
Jul 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·J KamholzR Lazzarini
Dec 31, 1997·Preparative Biochemistry & Biotechnology·K Guo, H H Berlet
Jan 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·N K ZellerR A Lazzarini
Oct 1, 1984·Neurochemical Research·N Kerlero De RosboD S Linthicum
Mar 1, 1984·Journal of Neurochemistry·A T CampagnoniN Baumann
Jan 1, 1987·Journal of Neuroscience Research·A T CampagnoniJ E Moskaitis

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