Nov 1, 1975

Preparation and properties of beta-galactosidase linked covalently with KM-cellulose

Prikladnaia biokhimiia i mikrobiologiia
S V EchitsE V Letunova

Abstract

Fungal beta-galactosidase was immobilized by covalent binding with KM-cellulose. The resultant preparation contained 3 mg protein per 1 g carrier; its specific activity was 65% of the initial one. As a result of immobilization pH optimum remained unchanged whereas the temperature optimum decreased from 65 degrees to 50 degrees. The seemings Km of the immobilized enzyme varied insignificantly as compared with Km of the soluble enzyme.

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Mentioned in this Paper

Covalent Interaction
GLB1
Filamentous fungus
PLIN1
Plasma Protein Binding Capacity
Soluble
Beta-Galactosidase
Fungi
Cellulose
Hydrogen-Ion Concentration

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