PMID: 5710Mar 1, 1976

Preparation and properties of poly 2'-O-ethylcytidylic acid

Nucleic Acids Research
M Kielanowska, D Shugar

Abstract

Poly 2'0-ethylcytidylic acid (poly (Ce)) was prepared by polymerization of 2'-0-ethylcytidine-5'-pyrophosphate with Escherichia coli polynucleotide phosphorylase in the presence of Mn++, and its properties compared with those of poly (rC), poly (Cm) and poly (dC). The neutral form of pOLY (Ce) exhibits properties similar to those of poly (rC) and poly (Cm). It also forms an acid twin-stranded helix with a transition pH of 5.9 in 0.1 M NaCl. The neutral form readily forms a double-stranded helical complex with poly (rI). Relative to poly (Cm), replacement of the 2'-0-methyl by 2-0-ethyl leads to increased enhancement of the thermal stabilities of both the acid helical form of poly (Ce) and its complex with poly (rI).

References

Jan 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·Y FuruichiA J Shatkin
Jul 1, 1969·Biochemistry·B ZmudzkaD Shugar
Nov 28, 1969·Journal of Molecular Biology·B ZmudzkaD Shugar
Aug 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·R W TennantJ G Farrelly
Jul 17, 1973·Biochemical and Biophysical Research Communications·J T KuśmierekD Shugar
Dec 4, 1969·Biochemical and Biophysical Research Communications·B ZmudzkaD Shugar
Apr 22, 1969·Biochimica Et Biophysica Acta·B G Lane, T Tamaoki
Mar 1, 1963·Biochemistry·E O AKINRIMISIP O TS'O
May 5, 1965·Journal of the American Chemical Society·K A HARTMAN, A RICH

Related Concepts

Enzyme Activation
Alkalescens-Dispar Group
Hydrogen-Ion Concentration
Manganese
RNA Conformation
RNA Denaturation
Genomic Hybridization
Poly C
Polyribonucleotide Nucleotidyltransferase
Polyribonucleotides

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