PMID: 16617789Apr 19, 2006Paper

Preparation and purification of polyclonal antibody against chymopapain

Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials
Yu-lan DingShu-jin Zhao

Abstract

To prepare and purify polyclonal antibody against chymopapain, and to make a foundation for establishing an immunossay for chymopapain. New Zealand rabbit was immunized with chymopapain. Antiserum was purified by Protein A and analyzed by ELISA. The titer of the antiserum obtained in this experiment by ELISA was up to 1:380000 and the purity was proved to be high by SDS-PAGE.

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