PMID: 15367350Sep 16, 2004Paper

Preparation and purification of the antibody against ribonuclease inhibitor

Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology
Xiao-hong ShuXiu-yun Cui

Abstract

To prepare and purify the antibody against ribonuclease inhibitor(RI). RI was extracted from human placenta and purified by affinity chromatography. Rabbits anti-RI antibody was obtained after immunization and then purified through rProtein A Sepharose Fast Flow chromatography column. The characters of the antibody was identified by SDS-PAGE, ELISA and Western blot. An anti-RI antibody was obtained and purified. SDS-PAGE analysis showed that the purified anti-RI antibody has high purity. The results of Western blot and ELISA indicated that anti-RI antibody had high specificity and good stability. The anti-RI antibody with high titer, high specificity and good stability has been acquired, which lays the foundation for further research on RI.

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