PMID: 3758463Jan 1, 1986Paper

Preparation and purification of the proteinase inhibitor, leupeptin, from culture filtrates of Streptomyces lavendulae

The International Journal of Biochemistry
M C Ning, R J Beynon

Abstract

Leupeptin (N-acetyl-L-leucyl-L-leucyl-L-argininal) was produced as an extracellular metabolite by Streptomyces lavendulae. Addition of lysine to the growth medium stimulated leupeptin production from 200 to 1400 micrograms/ml. Incubation of late exponential phase mycelium in a synthetic medium was used to prepare single labelled (3H) and double labelled (3H/14C) inhibitor for metabolic studies. An improved purification scheme that generates leupeptin of high purity with good recoveries is also reported.

References

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Aug 1, 1969·FEBS Letters·W Gross, K Ring

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