PMID: 6160208Dec 1, 1980Paper

Preparation and retrograde axonal transport of an antiviral drug/horseradish peroxidase conjugate

Journal of Neurochemistry
R H HaschkeA H Bunt

Abstract

Recurrent Herpes simplex virus infections of the cornea are currently treated with antiviral drugs administered locally during periods of active infection. We have examined the feasibility of an alternate approach to treating recurrent infection, that of delivering an antiviral drug by axonal transport to cell somata in the trigeminal ganglion where the latent virus is thought to reside. We have coupled an antiviral drug to horseradish peroxidase (HRP), a protein which is readily transported retrogradely in corneal sensory axons to ganglion cell somata in the trigeminal ganglion. The antiviral drug 5-iodo-5'-amino-2',5'-dideoxyuridine (AIDU) was synthesized with 125I and coupled to horseradish peroxidase by Schiff base formation between the 5' amino group and aldehydes generated on the carbohydrate of the HRP following short periodate oxidation. The useful ratio of AIDU:HRP was 2-9; higher ratios resulted in an insoluble product. Sodium dodecylsulfate (SDS) acrylamide gel electrophoresis of the drug-protein conjugate revealed considerable aggregation and the isoelectric point of HRP was changed from 8.1 to 4.4-5.8 following the coupling procedure. Despite evidence that the protein conformation was considerably altered, the spec...Continue Reading

References

Apr 1, 1976·Journal of Medicinal Chemistry·T S LinW H Prusoff
Nov 1, 1977·Archives of Ophthalmology·R G MartinJ O Oh
Mar 29, 1973·The New England Journal of Medicine·J R Baringer, P Swoveland
May 1, 1974·Infection and Immunity·M L CookJ G Stevens

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Citations

Jan 21, 2010·BMC Neuroscience·Aaron G FillerAndrew M L Lever
Oct 31, 2012·International Journal for Numerical Methods in Biomedical Engineering·A V Kuznetsov
Nov 1, 2016·Journal of Neurochemistry·Steven W Barger
Jun 1, 1981·Hospital Practice·R F Schinazi, W H Prusoff

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