PMID: 6411130Aug 23, 1983Paper

Preparation and some properties of a derivative of wheat germ agglutinin with altered biological activity

Biochimica Et Biophysica Acta
P GangulyR M Macleod

Abstract

An inactive derivative of wheat germ agglutinin, which is a strong activator of blood platelets, was prepared by selective chemical modification of the lectin with cyanogen bromide at acid pH. The derivative was then used as a probe to learn about the initial events in platelet stimulation by physiological agents. Amino acid analysis of the modified lectin confirmed specific cleavage of a methionine residue. Gel filtration studies indicated a molecular weight for the lectin derivative similar to the unmodified lectin. In gel electrophoresis in the presence of sodium dodecyl sulfate, reduced samples of the derivative showed two bands and the main component migrated slightly faster than the native lectin. The derivative retained the capacity to precipitate an antibody to the lectin although at least one of the antigenic sites was lost due to chemical modification. The derivative did not compete with the unmodified lectin for binding to platelets. Unlike the parent lectin, the derivative did not aggregate platelets even at a ten fold higher concentration. Under similar conditions, there were about 1.0 X 10(5) binding sites/platelet for the lectin derivative with an apparent dissociation constant of 1.7 microM compared to 5 X 10(5)...Continue Reading

References

Nov 27, 1979·Biochemistry·B P PetersM Flashner
Nov 1, 1978·The Journal of Clinical Investigation·B B WekslerE A Jaffe
Jul 1, 1979·European Journal of Biochemistry·M MonsignyE Boschetti
Jan 1, 1979·Thrombosis Research·P Ganguly, N L Gould
Aug 28, 1979·Biochemical and Biophysical Research Communications·P Ganguly, N G Fossett
Apr 25, 1977·Journal of Molecular Biology·C S Wright
Nov 2, 1976·Biochemistry·B M MartinT C Detwiler
Apr 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·G R GuntherG M Edelman
Jan 1, 1973·The Biochemical Journal·A K AllenN Sharon
Feb 1, 1974·Nature·P Ganguly
Mar 16, 1981·Biochemical and Biophysical Research Communications·P Ganguly, N G Fossett
Apr 1, 1980·The Journal of Cell Biology·W M Hall, P Ganguly

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Citations

Aug 1, 1991·Archives of Biochemistry and Biophysics·N K BalasubramaniamA G Rao

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